Biomaterials Nanotechnology and Tissue Engineering faculty, School of Advanced Medical Technology, Isfahan University of Medical Sciences, Isfahan, 8174673461, Iran.
Department of Nanotechnology Engineering, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, 81746-73441, Iran.
J Biomed Mater Res A. 2018 Aug;106(8):2181-2189. doi: 10.1002/jbm.a.36425. Epub 2018 Apr 30.
In this study, biodegradable nanocomposites consisting of poly (glycerol sebacate) (PGS) elastomeric matrix and the reinforcing phase of calcium titanate (CaTiO ) nanoparticles were fabricated as a nerve guidance conduit (NGC) for peripheral nerve regeneration. CaTiO nanoparticles were synthesized via the sol-gel method and calcined at 800°C for 60 min. PGS elastomer was synthesized via the polycondensation reaction of glycerol and sebacate (1:1) and 2.5 and 5 wt. percentages of the synthesized CaTiO nanoparticles were added to the PGS prepolymer solution. The composites obtained were heated in order to make crosslinks in the pre-polymer. CaTiO nanoparticles, PGS elastomer, and the composites fabricated were characterized in terms of their structural, chemical, physical, mechanical, and cell response properties to evaluate the feasibility of using the nanocomposite for NGC applications. The results indicated that CaTiO nanoparticles were 50 nm in size. When the nanoparticles were added to the PGS, the elastic modulus and tensile strength of the nanocomposite reached values of about 1 and 0.5 MPa, respectively that are near those of natural nerves. The degradation behavior and swelling of the nanocomposites, as compared with those of the PGS elastomer, were controlled by introducing CaTiO into the PGS, which swelling limitation could prevent nerve compression. It was observed that Ca ions established chemical bonds with PGS, which led to high crosslink densities that, in turn, contribute to improved mechanical properties of the composite. The Ca ions released from the nanocomposite samples were in the nontoxic range. The PC12 cell line on the surface of the nanocomposite specimens showed good cell adhesion and proliferation with improved axon outgrowth and extension. Based on the results obtained the fabricated PGS/CaTiO nanocomposite may be recommended as a suitable NGC with desirable effects on peripheral nerve regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2181-2189, 2018.
在这项研究中，制备了由聚（癸二酸丙二醇酯）（PGS）弹性体基质和增强相的钛酸钙（CaTiO ）纳米粒子组成的可生物降解纳米复合材料，用作周围神经再生的神经引导管（NGC）。CaTiO 纳米粒子通过溶胶-凝胶法合成，并在 800°C 下煅烧 60 分钟。PGS 弹性体通过甘油和癸二酸（1：1）的缩聚反应合成，并向 PGS 预聚物溶液中加入 2.5 和 5wt%的合成 CaTiO 纳米粒子。获得的复合材料在加热过程中进行交联。对 CaTiO 纳米粒子、PGS 弹性体和制备的复合材料进行了结构、化学、物理、机械和细胞响应特性的表征，以评估纳米复合材料用于 NGC 应用的可行性。结果表明，CaTiO 纳米粒子的尺寸为 50nm。当纳米粒子加入到 PGS 中时，纳米复合材料的弹性模量和拉伸强度分别达到约 1 和 0.5MPa，接近天然神经的数值。与 PGS 弹性体相比，纳米复合材料的降解行为和溶胀受到 CaTiO 的引入的控制，这可以防止神经压缩。观察到 Ca 离子与 PGS 形成化学键，导致交联密度增加，从而提高复合材料的机械性能。从纳米复合材料样品中释放的 Ca 离子处于无毒范围。PC12 细胞系在纳米复合材料样品表面表现出良好的细胞黏附和增殖，具有改善的轴突生长和延伸。基于获得的结果，制备的 PGS/CaTiO 纳米复合材料可以作为一种合适的 NGC，对周围神经再生具有理想的效果。©2018 年 Wiley 期刊，Inc.J 生物材料研究 A 部分：106A：2181-2189，2018。
