Bu Xiangmei, Wang Bo, Wang Yaoqi, Wang Zhigang, Gong Chunzhi, Qi Feng, Zhang Caixia
Department of Anesthesiology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.
Department of Anesthesiology, Binzhou Medical University Hospital, Binzhou, Shandong 256603, P.R. China.
Exp Ther Med. 2017 Jul;14(1):97-106. doi: 10.3892/etm.2017.4504. Epub 2017 May 23.
Off-pump coronary artery bypass graft (CABG) surgery has recently emerged as a means to avoid the sequelae of extracorporeal circulation, including the whole-body inflammatory response, coagulation disorders and multiple organ dysfunction. At present, gas anesthesia, sevoflurane and intravenous anesthesia and propofol have been widely used during the CABG. To further understand the underlying mechanisms of these anesthetics on the gene level, the present study conducted pathway-related module analysis based on a co-expression network. This was performed in order to identify significant pathways in coronary artery disease patients who had undergone off-pump CABG surgery before and after applying sevoflurane or propofol. A total of 269 and 129 differentially expressed genes were obtained in the sevoflurane and propofol groups, respectively. In total, eight and seven pathways (P<0.05) in the sevoflurane and propofol groups were separately obtained via Kyoto Encyclopedia of Genes and Genome pathway analysis. Finally, eight and seven pathway-related modules in the sevoflurane and propofol groups were obtained, respectively. Furthermore, the mean degree of complement and coagulation cascades pathway-related module in both of the groups was the highest. It was predicted that during the CABG, the anesthetics might activate the complement and coagulation systems in order to possess some cardioprotective properties.
非体外循环冠状动脉旁路移植术（CABG）最近已成为一种避免体外循环后遗症的方法，这些后遗症包括全身炎症反应、凝血障碍和多器官功能障碍。目前，气体麻醉、七氟醚以及静脉麻醉和丙泊酚已在CABG手术中广泛使用。为了在基因水平上进一步了解这些麻醉剂的潜在机制，本研究基于共表达网络进行了通路相关模块分析。这样做是为了确定在应用七氟醚或丙泊酚前后接受非体外循环CABG手术的冠心病患者中的显著通路。在七氟醚组和丙泊酚组中分别获得了269个和129个差异表达基因。通过京都基因与基因组百科全书通路分析，在七氟醚组和丙泊酚组中分别总共获得了8条和7条通路（P<0.05）。最后，在七氟醚组和丙泊酚组中分别获得了8个和7个与通路相关的模块。此外，两组中补体和凝血级联反应通路相关模块的平均度数最高。据推测，在CABG手术期间，这些麻醉剂可能会激活补体和凝血系统，从而具有一些心脏保护特性。
