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芦丁抑制糖尿病肾病中高糖诱导的ACTA2和p38蛋白表达。

Rutin suppresses high glucose-induced ACTA2 and p38 protein expression in diabetic nephropathy.

作者信息

Han Chun-Shan, Liu Kai, Zhang Ning, Li Shi-Wen, Gao Hai-Cheng

机构信息

Department of Chest Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.

Department of Pathology and Pathophysiology, Chengde Medical College, Chengde, Hebei 300000, P.R. China.

出版信息

Exp Ther Med. 2017 Jul;14(1):181-186. doi: 10.3892/etm.2017.4509. Epub 2017 May 23.

DOI:10.3892/etm.2017.4509
PMID:28672912
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5488511/
Abstract

The present study investigated the effect of rutin on high glucose-induced actin, α2, smooth muscle, aorta (ACTA2) and p38 protein expression in diabetic nephropathy (DN). Human mesangial cells were divided into a control group, high glucose-induced mesangial cell group, high glucose + captopril group, and high glucose + rutin group (low, middle and high doses of rutin). Cell viability, adenosine 5'-triphosphate (ATP) content, cell cycle, and ACTA2 and p38 protein expression were examined using MTT assay, ATP assay kit, flow cytometry and immunofluorescence staining in cultured human mesangial cells, respectively. Cell viability, ATP content, and ACTA2 and p38 expression increased significantly in high glucose-induced mesangial cells (P<0.05). However, at concentrations of 0.2, 0.4 and 0.8 µmol/l rutin was able to inhibit high glucose-induced human mesangial cell viability, ATP content, and ACTA2 and p38 expression and improve the cell cycle progression of mesangial cells. In conclusion, ACTA2 and p38 proteins may have important roles in DN. Rutin may inhibit the expression of ACTA2 and p38 and may be utilized in the prevention and treatment of DN.

摘要

本研究调查了芦丁对糖尿病肾病（DN）中高糖诱导的肌动蛋白、α2平滑肌肌动蛋白（ACTA2）和p38蛋白表达的影响。将人系膜细胞分为对照组、高糖诱导系膜细胞组、高糖+卡托普利组和高糖+芦丁组（低、中、高剂量芦丁）。分别采用MTT法、ATP检测试剂盒、流式细胞术和免疫荧光染色法检测培养的人系膜细胞的细胞活力、三磷酸腺苷（ATP）含量、细胞周期以及ACTA2和p38蛋白表达。高糖诱导的系膜细胞中细胞活力、ATP含量以及ACTA2和p38表达显著增加（P<0.05）。然而，在芦丁浓度为0.2、0.4和0.8µmol/l时，其能够抑制高糖诱导的人系膜细胞活力、ATP含量以及ACTA2和p38表达，并改善系膜细胞的细胞周期进程。总之，ACTA2和p38蛋白可能在DN中起重要作用。芦丁可能抑制ACTA2和p38的表达，并可用于DN的预防和治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a2b/5488511/16b2868d256d/etm-14-01-0181-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a2b/5488511/a73ca42b7ae5/etm-14-01-0181-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a2b/5488511/5c43f63fa5c3/etm-14-01-0181-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a2b/5488511/398dd191f27b/etm-14-01-0181-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a2b/5488511/a18c9aa4a0fc/etm-14-01-0181-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a2b/5488511/16b2868d256d/etm-14-01-0181-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a2b/5488511/a73ca42b7ae5/etm-14-01-0181-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a2b/5488511/5c43f63fa5c3/etm-14-01-0181-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a2b/5488511/398dd191f27b/etm-14-01-0181-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a2b/5488511/a18c9aa4a0fc/etm-14-01-0181-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a2b/5488511/16b2868d256d/etm-14-01-0181-g05.jpg

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