Institute for Molecular Bacteriology, TWINCORE GmbH, Centre for Experimental and Clinical Infection Research, a joint venture of the Hannover Medical School and the Helmholtz Centre for Infection Research, Hannover 30625, Germany.
Research Core Unit Proteomics and Institute of Toxicology, Hannover Medical School, Hannover 30625, Germany.
Cells. 2019 Sep 23;8(10):1129. doi: 10.3390/cells8101129.
Comparative genomics has greatly facilitated the identification of shared as well as unique features among individual cells or tissues, and thus offers the potential to find disease markers. While proteomics is recognized for its potential to generate quantitative maps of protein expression, comparative proteomics in bacteria has been largely restricted to the comparison of single cell lines or mutant strains. In this study, we used a data independent acquisition (DIA) technique, which enables global protein quantification of large sample cohorts, to record the proteome profiles of overall 27 whole genome sequenced and transcriptionally profiled clinical isolates of the opportunistic pathogen Analysis of the proteome profiles across the 27 clinical isolates grown under planktonic and biofilm growth conditions led to the identification of a core biofilm-associated protein profile. Furthermore, we found that protein-to-mRNA ratios between different strains are well correlated, indicating conserved patterns of post-transcriptional regulation. Uncovering core regulatory pathways, which drive biofilm formation and associated antibiotic tolerance in bacterial pathogens, promise to give clues to interactions between bacterial species and their environment and could provide useful targets for new clinical interventions to combat biofilm-associated infections.
比较基因组学极大地促进了个体细胞或组织之间共享和独特特征的识别，因此有潜力发现疾病标志物。尽管蛋白质组学因其能够生成蛋白质表达的定量图谱而受到认可，但细菌中的比较蛋白质组学在很大程度上仅限于单细胞系或突变株的比较。在这项研究中，我们使用了一种数据非依赖性采集 (DIA) 技术，该技术能够对大量样本队列进行全局蛋白质定量，记录了机会性病原体 27 个全基因组测序和转录谱分析的临床分离株的蛋白质组谱。对 27 株在浮游和生物膜生长条件下生长的临床分离株的蛋白质组谱进行分析，确定了一个核心生物膜相关蛋白谱。此外，我们发现不同菌株之间的蛋白质与 mRNA 比率之间存在很好的相关性，表明转录后调控模式具有保守性。揭示驱动细菌病原体生物膜形成和相关抗生素耐药性的核心调控途径，有望为细菌物种与其环境之间的相互作用提供线索，并为对抗生物膜相关感染的新临床干预措施提供有用的目标。
