Asimakis G K, Wilson D E, Conti V R
Life Sci. 1985 Dec 23;37(25):2373-80. doi: 10.1016/0024-3205(85)90104-3.
Release of AMP and adenosine from rat heart mitochondria was studied. The rate of appearance of extramitochondrial adenosine was independent of the extramitochondrial phosphate concentration between 5 and 20 mM. In the absence of exogenous, respiratory substrates or in the presence of glutamate/malate plus rotenone, the rate of appearance of adenosine was relatively low when phosphate was not added. The appearance of extramitochondrial AMP + adenosine was found to be directly proportional to the extra-mitochondrial phosphate concentration. Zn2+ (10 mM) decreased the rate of adenosine appearance by 90% and increased the rate of AMP appearance 6-fold. The mitochondrial preparations dephosphorylated exogenous AMP; this activity was inhibited by 10 mM Zn2+. We conclude that the adenosine appearing in the extramitochondrial space was not due to a direct release from the matrix, but instead was due to adenine nucleotide release with subsequent conversion to adenosine in the extramitochondrial space.
对大鼠心脏线粒体中AMP和腺苷的释放进行了研究。线粒体外腺苷的出现速率在5至20 mM的线粒体外磷酸盐浓度范围内与该浓度无关。在没有外源性呼吸底物的情况下，或在存在谷氨酸/苹果酸加鱼藤酮的情况下，当不添加磷酸盐时，腺苷的出现速率相对较低。发现线粒体外AMP + 腺苷的出现与线粒体外磷酸盐浓度成正比。10 mM的Zn2+使腺苷出现速率降低90%，并使AMP出现速率增加6倍。线粒体制剂使外源性AMP去磷酸化；该活性受到10 mM Zn2+的抑制。我们得出结论，出现在线粒体外空间的腺苷并非直接从基质中释放，而是由于腺嘌呤核苷酸释放，随后在线粒体外空间转化为腺苷。
