Cox Sherry, Bailey Joan, Okafor Chika, Seddighi Reza, Doherty Tom
Department of Biomedical and Diagnostic Sciences, University of Tennessee, Knoxville, TN, United States of America.
Department of Large Animal Clinical Sciences, University of Tennessee, Knoxville, TN, United States of America.
PeerJ. 2017 Jun 30;5:e3476. doi: 10.7717/peerj.3476. eCollection 2017.
Propofol is an intravenous anesthetic commonly used due to its favorable pharmacokinetic and pharmacodynamic profile. There are discrepancies in the literature about the most appropriate sample for determining propofol concentrations. Although plasma has been used for determining propofol concentrations, whole blood has been the preferred sample. There is also a lack of consistency in the literature on the effect of storage time and temperature on propofol concentrations and this may lead to errors in the design of pharmacokinetic/pharmacodynamics studies. The purpose of this study was to determine the difference in propofol concentrations in whole blood versus plasma and to evaluate the influence of storage time (56 days) and temperature (4 °C, -20 °C, -80 °C) on the stability of propofol concentrations in blood and plasma samples. Results from the study indicate that whole blood and plasma samples containing propofol stored at -80 °C have concentrations as high as or higher than those stored at 4 °C or -20 °C for 56 days; thus, -80 °C is an appropriate temperature for propofol sample storage. Plasma propofol concentrations were consistently higher than whole blood for all three storage temperatures. Consequently, plasma is the most appropriate sample for propofol analysis due to its consistent determinations.
丙泊酚是一种常用的静脉麻醉剂，因其良好的药代动力学和药效学特性而被广泛应用。关于测定丙泊酚浓度最合适的样本，文献中存在差异。虽然血浆已被用于测定丙泊酚浓度，但全血一直是首选样本。关于储存时间和温度对丙泊酚浓度的影响，文献中也缺乏一致性，这可能导致药代动力学/药效学研究设计出现错误。本研究的目的是确定全血与血浆中丙泊酚浓度的差异，并评估储存时间（56天）和温度（4℃、-20℃、-80℃）对血液和血浆样本中丙泊酚浓度稳定性的影响。研究结果表明，在-80℃储存的含丙泊酚全血和血浆样本的浓度与在4℃或-20℃储存56天的样本浓度一样高或更高；因此，-80℃是丙泊酚样本储存的适宜温度。在所有三种储存温度下，血浆丙泊酚浓度始终高于全血。因此，由于血浆测定结果一致，它是丙泊酚分析最合适的样本。
