Barrio Lara, Milán Marco
Institute for Research in Biomedicine (IRB Barcelona), Barcelona, Spain.
The Barcelona Institute of Science and Technology (BIST), Barcelona, Spain.
Elife. 2017 Jul 4;6:e22013. doi: 10.7554/eLife.22013.
The gradient of Decapentaplegic (Dpp) in the wing has served as a paradigm to characterize the role of morphogens in regulating patterning. However, the role of this gradient in regulating tissue size is a topic of intense debate as proliferative growth is homogenous. Here, we combined the Gal4/UAS system and a temperature-sensitive Gal80 molecule to induce RNAi-mediated depletion of and characterise the spatial and temporal requirement of Dpp in promoting growth. We show that Dpp emanating from the AP compartment boundary is required throughout development to promote growth by regulating cell proliferation and tissue size. Dpp regulates growth and proliferation rates equally in central and lateral regions of the developing wing appendage and reduced levels of Dpp affects similarly the width and length of the resulting wing. We also present evidence supporting the proposal that graded activity of Dpp is not an absolute requirement for wing growth.
翅中Decapentaplegic（Dpp）梯度已成为表征形态发生素在调控模式形成中作用的范例。然而，由于增殖生长是均匀的，该梯度在调控组织大小方面的作用是一个激烈争论的话题。在这里，我们结合Gal4/UAS系统和温度敏感的Gal80分子，诱导RNAi介导的[具体基因名称缺失]缺失，并表征Dpp在促进生长中的时空需求。我们表明，从前后（AP）隔室边界发出的Dpp在整个发育过程中都是必需的，它通过调节细胞增殖和组织大小来促进生长。Dpp在发育中的翅附属器的中央和外侧区域同等程度地调节生长和增殖速率，Dpp水平降低对所得翅膀的宽度和长度有类似影响。我们还提供了证据支持以下提议，即Dpp的梯度活性并非翅膀生长的绝对必要条件。
