Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, 1417755469, Iran.
Nano Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, 1435944711, Iran.
J Biomed Mater Res B Appl Biomater. 2018 May;106(4):1463-1476. doi: 10.1002/jbm.b.33952. Epub 2017 Jul 4.
The current study aimed to enhance the efficacy of peripheral nerve regeneration using an electrically conductive biodegradable porous neural guidance conduit for transplantation of allogeneic Schwann cells (SCs). The conduit was produced from polylactic acid (PLA), multiwalled carbon nanotubes (MWCNTs), and gelatin nanofibrils (GNFs) coated with the recombinant human erythropoietin-loaded chitosan nanoparticles (rhEpo-CNPs). The PLA/MWCNTs/GNFs/rhEpo-CNPs conduit had the porosity of 85.78 ± 0.70%, the contact angle of 77.65 ± 1.91° and the ultimate tensile strength and compressive modulus of 5.51 ± 0.13 MPa and 2.66 ± 0.34 MPa, respectively. The conduit showed the electrical conductivity of 0.32 S cm and lost about 11% of its weight after 60 days in normal saline. The produced conduit was able to release the rhEpo for at least 2 weeks and exhibited favorable cytocompatibility towards SCs. For functional analysis, the conduit was seeded with 1.5 × 10 SCs and implanted into a 10 mm sciatic nerve defect of Wistar rat. After 14 weeks, the results of sciatic functional index, hot plate latency, compound muscle action potential amplitude, weight-loss percentage of wet gastrocnemius muscle and Histopathological examination using hematoxylin-eosin and Luxol fast blue staining demonstrated that the produced conduit had comparable nerve regeneration to the autograft, as the gold standard to bridge the nerve gaps. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1463-1476, 2018.
本研究旨在通过使用导电可生物降解多孔神经引导导管来增强周围神经再生的效果，该导管用于移植同种异体雪旺细胞（SCs）。该导管由聚乳酸（PLA）、多壁碳纳米管（MWCNTs）和涂有重组人促红细胞生成素负载壳聚糖纳米颗粒（rhEpo-CNPs）的明胶纳米原纤维（GNFs）制成。PLA/MWCNTs/GNFs/rhEpo-CNPs 导管的孔隙率为 85.78±0.70%，接触角为 77.65±1.91°，拉伸强度和压缩模量分别为 5.51±0.13 MPa 和 2.66±0.34 MPa。导管的电导率为 0.32 S cm，在生理盐水环境中放置 60 天后，其重量损失约为 11%。所制备的导管能够持续释放 rhEpo 至少 2 周，并且对 SCs 具有良好的细胞相容性。为了进行功能分析，将 1.5×10 的 SCs 接种到 10mm 坐骨神经缺损的 Wistar 大鼠中，植入导管。14 周后，通过坐骨神经功能指数、热板潜伏期、复合肌肉动作电位幅度、湿腓肠肌重量损失百分比和苏木精-伊红及卢索快速蓝染色的组织病理学检查，结果表明该导管具有与自体移植物相当的神经再生效果，可作为桥接神经间隙的金标准。©2017 Wiley Periodicals, Inc. J 生物材料研究杂志 B：应用生物材料，106B：1463-1476，2018。
