Nabipour Mehran, Mellati Amir, Abasi Mozhgan, Barough Somayeh Ebrahimi, Karimizade Ayoob, Banikarimi Parnian, Hasanzadeh Elham
Department of Tissue Engineering & Regenerative Medicine, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Student Research Committee, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
J Biol Eng. 2024 Feb 22;18(1):16. doi: 10.1186/s13036-024-00412-9.
Due to loss of peripheral nerve structure and/or function resulting from trauma, accidents, and other causes, peripheral nerve injuries continue to be a major clinical problem. These injuries can cause partial or total loss of sensory, motor, and autonomic capabilities as well as neuropathic pain. PNI affects between 13 and 23 out of every 100,000 people annually in developed countries. Regeneration of damaged nerves and restoration of function after peripheral nerve injury remain significant therapeutic challenges. Although autologous nerve graft transplantation is a viable therapy option in several clinical conditions, donor site morbidity and a lack of donor tissue often hinder full functional recovery. Biomimetic conduits used in tissue engineering to encourage and direct peripheral nerve regeneration by providing a suitable microenvironment for nerve ingrowth are only one example of the cutting-edge methods made possible by this field. Many innate extracellular matrix (ECM) structures of different tissues can be successfully mimicked by nanofibrous scaffolds. Nanofibrous scaffolds can closely mimic the surface structure and morphology of native ECMs of many tissues.
In this study, we have produced bilayer nanofibrous nerve conduit based on poly-lactic acid/polyurethane/multiwall carbon nanotube (PLA/PU/MWCNT), for application as composite scaffolds for static nerve tissue engineering. The contact angle was indicated to show the hydrophilicity properties of electrospun nanofibers. The SEM images were analyzed to determine the fiber's diameters, scaffold morphology, and endometrial stem cell adhesion. Moreover, MTT assay and DAPI staining were used to show the viability and proliferation of endometrial stem cells.
The constructed bilayer PLA/PU/MWCNT scaffolds demonstrated the capacity to support cell attachment, and the vitality of samples was assessed using SEM, MTT assay, and DAPI staining technique.
According to an in vitro study, electrospun bilayer PLA/PU/MWCNT scaffolds can encourage the adhesion and proliferation of human endometrial stem cells (hEnSCs) and create the ideal environment for increasing cell survival.
由于创伤、事故及其他原因导致外周神经结构和/或功能丧失，外周神经损伤仍然是一个主要的临床问题。这些损伤可导致感觉、运动和自主神经功能部分或全部丧失以及神经性疼痛。在发达国家，每年每10万人中就有13至23人受到外周神经损伤（PNI）影响。外周神经损伤后受损神经的再生和功能恢复仍然是重大的治疗挑战。尽管自体神经移植在几种临床情况下是一种可行的治疗选择，但供体部位的发病率和供体组织的缺乏常常阻碍功能的完全恢复。组织工程中使用的仿生导管通过为神经向内生长提供合适的微环境来促进和引导外周神经再生，这只是该领域实现的前沿方法之一。不同组织的许多天然细胞外基质（ECM）结构可以被纳米纤维支架成功模拟。纳米纤维支架可以紧密模拟许多组织天然ECM的表面结构和形态。
在本研究中，我们制备了基于聚乳酸/聚氨酯/多壁碳纳米管（PLA/PU/MWCNT）的双层纳米纤维神经导管，用作静态神经组织工程的复合支架。通过接触角来表明电纺纳米纤维的亲水性。分析扫描电子显微镜（SEM）图像以确定纤维直径、支架形态和子宫内膜干细胞粘附情况。此外，采用MTT法和4',6-二脒基-2-苯基吲哚（DAPI）染色来显示子宫内膜干细胞的活力和增殖情况。
构建的双层PLA/PU/MWCNT支架表现出支持细胞附着的能力，并使用SEM、MTT法和DAPI染色技术评估了样本的活力。
根据一项体外研究，电纺双层PLA/PU/MWCNT支架可促进人子宫内膜干细胞（hEnSCs）的粘附和增殖，并为提高细胞存活率创造理想环境。
