使用尺寸控制免疫捕获芯片对循环肿瘤细胞进行分离、检测和基于抗原的分析。
Isolation, Detection, and Antigen-Based Profiling of Circulating Tumor Cells Using a Size-Dictated Immunocapture Chip.
机构信息
MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical and Biochemical Engineering, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
Department of Gastroenterology, Zhongshan Hospital, Xiamen University, Xiamen, 361005, China.
出版信息
Angew Chem Int Ed Engl. 2017 Aug 28;56(36):10681-10685. doi: 10.1002/anie.201702675. Epub 2017 Jul 26.
Even though the diagnostic and prognostic value of circulating tumor cells (CTCs) has been demonstrated, their clinical utility and widespread adoption have been limited. Herein, we describe a new device, size-dictated immunocapture chip (SDI-Chip), for efficient, sensitive, and spatially resolved capture and detection of CTCs. SDI-Chip enables selective, frequent, and extended interaction of CTCs with hydrodynamically optimized immunocoated micropillar surfaces. CTCs with different antigen expression levels can be efficiently captured and spatially resolved around the micropillars. Capture efficiency greater than 92 % with a purity of 82 % was achieved with blood samples. CTCs were detected in non-metastasis colorectal (CRC) patients, while none was detected from healthy volunteers. We believe that SDI-Chip will facilitate the transition of tumor diagnosis from anatomical pathology to molecular pathology in localized CRC patients.
虽然循环肿瘤细胞（CTCs）的诊断和预后价值已经得到证实，但它们的临床实用性和广泛应用受到了限制。在此，我们描述了一种新的设备，尺寸决定免疫捕获芯片（SDI-Chip），用于高效、敏感和空间分辨捕获和检测 CTCs。SDI-Chip 能够选择性、频繁地和扩展地与经流体动力学优化的免疫涂层微柱表面进行 CTC 相互作用。具有不同抗原表达水平的 CTCs 可以在微柱周围有效地被捕获和空间分辨。血液样本的捕获效率大于 92%，纯度为 82%。在非转移性结直肠癌（CRC）患者中检测到 CTCs，而在健康志愿者中则未检测到。我们相信，SDI-Chip 将促进肿瘤诊断从解剖病理学向局部 CRC 患者的分子病理学转变。
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