利用CRISPR/Cas9技术构建Bag1纯合敲除小鼠胚胎干细胞系。
Generation of a Bag1 homozygous knockout mouse embryonic stem cell line using CRISPR/Cas9.
作者信息
Tang Cheng-Cheng, Shan Li Pik, Wang Wu-Ming, Lu Gang, Tare Rahul S, Lee Kenneth Ka Ho
机构信息
MOE Key Laboratory for Regenerative Medicine, School of Biomedical Sciences, Chinese University of Hong Kong, Hong Kong, People's Republic of China; Chinese University of Hong Kong - University of Southampton Joint Laboratory for Regenerative Medicine, School of Biomedical Sciences, Chinese University of Hong Kong, Hong Kong, People's Republic of China.
Transgenic Core Service Centre, School of Biomedical Science, Chinese University of Hong Kong, Hong Kong, People's Republic of China.
出版信息
Stem Cell Res. 2017 May;21:29-31. doi: 10.1016/j.scr.2017.03.016. Epub 2017 Mar 23.
Bag1 transcribes a multifunctional protein that participates in many important biological processes such as cell apoptosis, proliferation, differentiation and embryo development. Despite numerous published studies, the role of Bag1 in the context of embryonic stem (ES) cells, has not been explored. To investigate the function of Bag1 in ES cells, we generated mutant Bag1 ES cells using the CRISPR/Cas9 system. We established that the Bag1 double knockout ES cell line maintained their pluripotency, possessed a normal karyotype and the ability to differentiate into all three germ layers.
Bag1转录一种多功能蛋白，该蛋白参与许多重要的生物学过程，如细胞凋亡、增殖、分化和胚胎发育。尽管已有大量研究发表，但Bag1在胚胎干细胞（ES细胞）中的作用尚未得到探索。为了研究Bag1在ES细胞中的功能，我们使用CRISPR/Cas9系统生成了Bag1突变型ES细胞。我们证实，Bag1双敲除ES细胞系保持了其多能性，具有正常的核型以及分化为所有三个胚层的能力。
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