Anzhen Hospital, Capital Medical University, Beijing 100029, China.
Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China.
Stem Cell Res. 2021 Aug;55:102471. doi: 10.1016/j.scr.2021.102471. Epub 2021 Jul 20.
In order to determine the function of dHAND in a specific subset of cardiomyocyte progenitor cells responsible for the heart and its function in the cellular mechanism of Hand2-/- mouse ventricular hypoplasia, we established an allelic knockout model of dHAND in human embryonic stem cells (hESCs-H9) by an episomal vector-based CRISPR/Cas9 system. This dHAND KO hESC line maintained normal karyotype and typical primed pluripotent human stem cell morphology, and maintained pluripotency, could differentiate into all three germ layers in vivo.
为了确定 dHAND 在负责心脏及其功能的特定心肌祖细胞亚群中的功能，以及在 Hand2-/- 小鼠心室发育不全的细胞机制中的作用，我们通过基于附加体载体的 CRISPR/Cas9 系统在人胚胎干细胞（hESCs-H9）中建立了 dHAND 的等位基因敲除模型。该 dHAND KO hESC 系保持正常核型和典型的初始多能人类干细胞形态，并且保持多能性，能够在体内分化为所有三个胚层。
