Mi Te, Luo Changjiang, Hu Yawei, Qu Chuanqiang, Wang Xiang, Guo Shougang, Du Yifeng
Department of ICU, Jining No. 1 People's Hospital, Jining, Shandong 272000, P.R. China.
Department of Neurology, Provincial Hospital Affiliated to Shandong University (South Branch), Jinan, Shandong 250002, P.R. China.
Mol Med Rep. 2017 Sep;16(3):2563-2569. doi: 10.3892/mmr.2017.6871. Epub 2017 Jun 28.
Circular RNAs (circRNAs) are class of endogenous RNAs that have a role in the regulation of gene expression. The present study aimed to investigate the diagnostic value and role of circRNA in the pathogenesis of leukoaraiosis (LA). The present study performed Arraystar Human circRNA Array analysis of 6 samples from LA cases and 6 samples from control cases. Differentially expressed (DE) circRNAs between two samples were identified through fold‑change (>1.5‑fold) screening. Afterwards, based on DE circRNAs, the gene ontology (GO) analysis of upregulated DE genes identified from DE circRNAs demonstrated that DE genes were primarily associated with cellular metabolic processes, membrane‑bound organelles and binding. However, none were enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Downregulated DE genes were enriched in cellular localization, cytoplasm and kinase binding. For the KEGG pathways, the downregulated DE genes were primarily associated with the insulin signaling pathway. The results of the present study indicated that the DE genes from differently expressed circRNAs may have an important role in the pathogenesis of LA and may be a novel targfet for further research.
环状RNA（circRNAs）是一类内源性RNA，在基因表达调控中发挥作用。本研究旨在探讨环状RNA在脑白质疏松症（LA）发病机制中的诊断价值及作用。本研究对6例LA病例样本和6例对照病例样本进行了Arraystar人类环状RNA芯片分析。通过倍数变化（>1.5倍）筛选鉴定出两个样本之间差异表达的（DE）环状RNA。之后，基于差异表达的环状RNA，对从差异表达环状RNA中鉴定出的上调差异表达基因进行基因本体（GO）分析，结果表明差异表达基因主要与细胞代谢过程、膜结合细胞器和结合相关。然而，在京都基因与基因组百科全书（KEGG）通路中均未富集。下调的差异表达基因在细胞定位、细胞质和激酶结合方面富集。对于KEGG通路，下调的差异表达基因主要与胰岛素信号通路相关。本研究结果表明，差异表达环状RNA中的差异表达基因可能在LA发病机制中起重要作用，可能是进一步研究的新靶点。
