Zhao WeiKang, Li Yuling, Zhou Ao, Chen Xiaojun, Li Kai, Chen Sinan, Qiao Bo, Jiang Dianming
Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Chongqing, Yuzhong District 400016, People's Republic of China.
Department of Orthopaedics, Third Affiliated Hospital of Chongqing Medical University, No. 1 Shuanghu Road, Chongqing City, Yubei District 401120, People's Republic of China.
R Soc Open Sci. 2020 Apr 1;7(4):191830. doi: 10.1098/rsos.191830. eCollection 2020 Apr.
Self-assembled peptide scaffolds based on D-RADA16 are an important matrix for controlled drug release and three-dimensional cell culture. In this work, D-RADA16 peptide hydrogels were coated on artificial bone composed of nano-hydroxyapatite/polyamide 66 (nHA/PA66) to obtain a porous drug-releasing structure for treating bone defects. The developed materials were characterized via transmission electron microscopy and scanning electron microscopy. The proliferation and adhesion of bone mesenchymal stem cells (BMSCs) were examined by confocal laser microscopy and CCK-8 experiments. The osteogenic ability of the porous materials towards bone BMSCs was examined by staining with Alizarin Red S and alkaline phosphatase, and bioactivity was evaluated . The results revealed that nHA/PA66/D-RADA16/bFGF reduces the degradation rate of D-RADA16 hydrogels and prolongs sustained release of bFGF, which would promote BMSCs proliferation, adhesion and osteogenesis and bone repair . Thus, it deserves more attention and is worthy of further research.
基于D-RADA16的自组装肽支架是用于可控药物释放和三维细胞培养的重要基质。在这项工作中，将D-RADA16肽水凝胶涂覆在由纳米羟基磷灰石/聚酰胺66（nHA/PA66）组成的人工骨上，以获得用于治疗骨缺损的多孔药物释放结构。通过透射电子显微镜和扫描电子显微镜对所开发的材料进行表征。通过共聚焦激光显微镜和CCK-8实验检测骨间充质干细胞（BMSC）的增殖和粘附。通过茜素红S和碱性磷酸酶染色检查多孔材料对骨BMSC的成骨能力，并评估生物活性。结果表明，nHA/PA66/D-RADA16/bFGF降低了D-RADA16水凝胶的降解速率，延长了bFGF的持续释放，这将促进BMSC的增殖、粘附和成骨以及骨修复。因此，它值得更多关注并值得进一步研究。
