Schäfer G, Schnellbacher-Daum G, Heyer A E, Schatz H
Diabetes Res. 1985 Jul;2(4):201-5.
This study deals with the influence insulin exerts upon pancreatic somatostatin release. In different incubation systems, isolated rat islets were subjected to addition of either exogenous rat insulin or of anti-insulin serum (AIS). No change in somatostatin release was caused by insulin (25 U/l) during perifusion of freshly isolated islets at 8.3 mmol/l glucose, whereas AIS showed an inhibitory effect. During a 42 hr culture period, somatostatin content of culture medium remained low in the presence of insulin but was elevated when AIS has been added. Therefore, further experiments were carried out after a 2-day period of precultivation. Precultivated islets responded to exogenous insulin with a decrease in somatostatin release in static incubations and in perifusion experiments. It is suggested that the lack of insulin effect in freshly isolated islets is due to alterations in receptors which are restored during islet culture. The inhibition of somatostatin release from freshly isolated islets observed during perifusion with AIS may be caused by anti-insulin receptor antibodies probably present in the anti-insulin serum.
本研究探讨胰岛素对胰腺生长抑素释放的影响。在不同的孵育系统中，将分离的大鼠胰岛分别加入外源性大鼠胰岛素或抗胰岛素血清（AIS）。在8.3 mmol/l葡萄糖条件下对新鲜分离的胰岛进行灌流时，胰岛素（25 U/l）未引起生长抑素释放的变化，而AIS则显示出抑制作用。在42小时的培养期内，培养基中的生长抑素含量在有胰岛素存在时保持较低水平，但加入AIS后则升高。因此，在预培养2天后进行了进一步的实验。预培养的胰岛在静态孵育和灌流实验中对外源性胰岛素的反应是生长抑素释放减少。提示新鲜分离的胰岛中胰岛素效应的缺乏是由于受体的改变，而这种改变在胰岛培养过程中得以恢复。在灌流AIS时观察到的新鲜分离胰岛生长抑素释放的抑制可能是由抗胰岛素血清中可能存在的抗胰岛素受体抗体引起的。
