Niklaus Stephanie, Neuhauss Stephan C F
a Institute of Molecular Life Sciences , University of Zurich , Zurich , Switzerland.
b Life Science Zurich Graduate Program - Neuroscience , Zurich , Switzerland.
J Neurogenet. 2017 Sep;31(3):70-87. doi: 10.1080/01677063.2017.1343316. Epub 2017 Jul 5.
The zebrafish (Danio rerio) possesses a vertebrate-type retina that is extraordinarily conserved in evolution. This well-organized and anatomically easily accessible part of the central nervous system has been widely investigated in zebrafish, promoting general understanding of retinal development, morphology, function and associated diseases. Over the recent years, genome and protein engineering as well as imaging techniques have experienced revolutionary advances and innovations, creating new possibilities and methods to study zebrafish development and function. In this review, we focus on some of these emerging technologies and how they may impact retinal research in the future. We place an emphasis on genetic techniques, such as transgenic approaches and the revolutionizing new possibilities in genome editing.
斑马鱼（Danio rerio）拥有一个在进化过程中极其保守的脊椎动物类型的视网膜。这个组织良好且在解剖学上易于接近的中枢神经系统部分，已在斑马鱼中得到广泛研究，推动了对视网膜发育、形态、功能及相关疾病的普遍理解。近年来，基因组和蛋白质工程以及成像技术取得了革命性的进展和创新，为研究斑马鱼的发育和功能创造了新的可能性和方法。在本综述中，我们重点关注其中一些新兴技术以及它们未来可能如何影响视网膜研究。我们着重介绍遗传技术，如转基因方法以及基因组编辑中带来变革的新可能性。
