Lemes Susy R, Chaves Dwight A, Silva Nelson J DA, Carneiro Cristiene C, Chen-Chen Lee, Almeida Luciane M DE, Gonçalves Pablo J, Melo-Reis Paulo R DE
Laboratório de Estudos Experimentais e Biotecnológicos, Pontifícia Universidade Católica de Goiás/PUC, Rua 232, 128, 3º andar, Sala 5, 74605-010 Goiânia, GO, Brazil.
Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Goiás/UFG, Campus-II, Avenida Esperança, s/n, Campus Samambaia, 74690-900 Goiânia, GO, Brazil.
An Acad Bras Cienc. 2017;89(3 Suppl):2043-2051. doi: 10.1590/0001-3765201720150797. Epub 2017 Jun 29.
The aim of this study was to evaluate the possible protective of C. guianensis oil against MMC and CP, which are direct- and indirect-acting chemical mutagens, using the micronucleus test. Three experiments were performed. First the C. guianensis oil was co-administered to mice at doses of 250, 500 and 1000 mg/kg bw with 4 mg/kg bw MMC or 50 mg/kg bw CP. Second, the mutagenic drug (CP) was administered ip 50 mg/kg bw and after 6 and 12 hours 250 and 500 mg/kg bw of C. guianensis oil were administered. In the last, C. guianensis oil was administrated (250 and 500 mg/kg bw) during five days and after it was administered ip 50 mg/kg bw CP. The results obtained showed that the C. guianensis oil is not cytotoxic neither genotoxic to mouse bone marrow. Regarding the antimutagenic effect, all doses of C. guianensis oil were significantly (p < 0.05) effective in reducing the frequency of micronucleated polychromatic erythrocytes, when compared with MMC or CP alone. Based on these results, our results suggest that the C. guianensis oil shows medicinal potential as an antimutagenic agent, modulating the mutagenicity caused by both direct- and indirect-acting chemical mutagens, in a mammalian model.
本研究的目的是使用微核试验评估圭亚那肉桂油对丝裂霉素C（MMC）和环磷酰胺（CP）（分别为直接作用和间接作用的化学诱变剂）可能的保护作用。进行了三项实验。首先，将圭亚那肉桂油以250、500和1000mg/kg体重的剂量与4mg/kg体重的MMC或50mg/kg体重的CP共同给予小鼠。其次，腹腔注射50mg/kg体重的诱变药物（CP），并在6小时和12小时后分别给予250和500mg/kg体重的圭亚那肉桂油。最后，连续五天给予圭亚那肉桂油（250和500mg/kg体重），之后腹腔注射50mg/kg体重的CP。所得结果表明，圭亚那肉桂油对小鼠骨髓既无细胞毒性也无遗传毒性。关于抗诱变作用，与单独使用MMC或CP相比，所有剂量的圭亚那肉桂油在降低多染性红细胞微核频率方面均具有显著效果（p<0.05）。基于这些结果，我们的研究结果表明，在哺乳动物模型中，圭亚那肉桂油作为一种抗诱变剂具有药用潜力，可调节直接作用和间接作用化学诱变剂引起的诱变性。
