Kerr Emily O, Dunham Maitreya J
Department of Genome Sciences, University of Washington, Seattle, Washington 98195.
Department of Genome Sciences, University of Washington, Seattle, Washington 98195
Cold Spring Harb Protoc. 2017 Jul 5;2017(7):pdb.prot089003. doi: 10.1101/pdb.prot089003.
The use of chemostat culture facilitates the careful comparison of different yeast strains growing in well-defined conditions. Variations in physiology can be measured by examining gene expression, metabolite levels, protein content, and cell morphology. In this protocol, we show how a combination of sample types can be collected during harvest from a single 20-mL chemostat in a ministat array, with special attention to coordinating the handling of the most time-sensitive sample types.
恒化器培养的使用有助于在明确界定的条件下对不同酵母菌株进行仔细比较。通过检查基因表达、代谢物水平、蛋白质含量和细胞形态可以测量生理变化。在本方案中，我们展示了如何在从微型培养器阵列中的单个20毫升恒化器收获期间收集多种类型的样品，特别注意协调对最具时间敏感性的样品类型的处理。
