Dunham Maitreya J, Kerr Emily O, Miller Aaron W, Payen Celia
Department of Genome Sciences, University of Washington, Seattle, Washington 98195
Department of Genome Sciences, University of Washington, Seattle, Washington 98195.
Cold Spring Harb Protoc. 2017 Jul 5;2017(7):pdb.top077610. doi: 10.1101/pdb.top077610.
Continuous culture provides many benefits over the classical batch style of growing yeast cells. Steady-state cultures allow for precise control of growth rate and environment. Cultures can be propagated for weeks or months in these controlled environments, which is important for the study of experimental evolution. Despite these advantages, chemostats have not become a highly used system, in large part because of their historical impracticalities, including low throughput, large footprint, systematic complexity, commercial unavailability, high cost, and insufficient protocol availability. However, we have developed methods for building a relatively simple, low-cost, small footprint array of chemostats that can be run in multiples of 32. This "ministat array" can be applied to problems in yeast physiology and experimental evolution.
与传统的分批培养酵母细胞的方式相比，连续培养具有许多优势。稳态培养能够精确控制生长速率和环境。在这些可控环境中，培养物可以繁殖数周或数月，这对于实验进化研究非常重要。尽管有这些优点，但恒化器尚未成为一种广泛使用的系统，很大程度上是因为其历史上存在的不实用性，包括通量低、占地面积大、系统复杂、商业上无法获得、成本高以及缺乏足够的操作方案。然而，我们已经开发出了构建相对简单、低成本、小占地面积的恒化器阵列的方法，该阵列可以以32的倍数运行。这种“微型恒化器阵列”可应用于酵母生理学和实验进化方面的问题。
