Payen Celia, Dunham Maitreya J
Department of Genome Sciences, University of Washington, Seattle, Washington 98195.
Department of Genome Sciences, University of Washington, Seattle, Washington 98195
Cold Spring Harb Protoc. 2017 Jul 5;2017(7):pdb.prot089011. doi: 10.1101/pdb.prot089011.
Experimental evolution is one approach used to address a broad range of questions related to evolution and adaptation to strong selection pressures. Experimental evolution of diverse microbial and viral systems has routinely been used to study new traits and behaviors and also to dissect mechanisms of rapid evolution. This protocol describes the practical aspects of experimental evolution with yeast grown in chemostats, including the setup of the experiment and sampling methods as well as best laboratory and record-keeping practices.
实验进化是一种用于解决与进化以及适应强大选择压力相关的广泛问题的方法。各种微生物和病毒系统的实验进化经常被用于研究新的性状和行为，以及剖析快速进化的机制。本方案描述了在恒化器中培养酵母进行实验进化的实际操作，包括实验设置、采样方法以及最佳实验室操作和记录保存方法。
