Miller Aaron W, Kerr Emily O, Dunham Maitreya J
Department of Genome Sciences, University of Washington, Seattle, Washington 98195.
Department of Genome Sciences, University of Washington, Seattle, Washington 98195
Cold Spring Harb Protoc. 2017 Jul 5;2017(7):pdb.prot088997. doi: 10.1101/pdb.prot088997.
Here, we describe instructions for the assembly of an array of miniature (20-mL) chemostats or "ministats" built from relatively inexpensive off-the-shelf parts. In experiments with yeast cultures, we have observed reproducibility in cellular physiology, gene expression patterns, and evolutionary outcomes with different ministats as well as between ministats and commercial large-volume platforms. Growth in continuous culture is a primary means for the characterization of yeast steady-state physiology, competition between strains, and long-term evolution experiments. We hope that these relatively inexpensive and high-throughput devices make the advantages of continuous culture growth more accessible to researchers.
在此，我们描述了一种由相对便宜的现成部件构建的微型（20毫升）恒化器阵列或“微型恒化器”的组装说明。在酵母培养实验中，我们观察到不同微型恒化器之间以及微型恒化器与商业大容量平台之间在细胞生理学、基因表达模式和进化结果方面具有可重复性。连续培养中的生长是表征酵母稳态生理学、菌株间竞争和长期进化实验的主要手段。我们希望这些相对便宜且高通量的设备能让研究人员更容易获得连续培养生长的优势。
