Lee S W, Way A W, Osen E G
Infect Immun. 1986 Feb;51(2):586-93. doi: 10.1128/iai.51.2.586-593.1986.
Pili were isolated and purified from Bordetella bronchiseptica. Electron microscopic observations revealed that pili are ubiquitous in this species. The occurrence of pili and flagella appeared to correlate with growth phase and colonial morphology. Pili were about 3 to 4 nm in diameter and morphologically similar to pili isolated from other gram-negative bacteria. Internal core structure was not evident. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified pili showed that up to three different pilus subunit variants could be observed on a single strain, depending on the colonial phase and culture condition. Enzyme immunoassay and immunoblot, however, showed that these subunit variants are serologically related. Mice vaccinated with purified pili were protected against a virulent intraperitoneal challenge of B. bronchiseptica. B. bronchiseptica pili were also found to be similar to Bordetella pertussis pili in morphology and in the molecular size and antigenic structure of pilus subunits. The intact pili of B. bronchiseptica and B. pertussis, however, appeared to have weak serological cross-reactivity.
从支气管败血波氏杆菌中分离并纯化菌毛。电子显微镜观察显示菌毛在该菌种中普遍存在。菌毛和鞭毛的出现似乎与生长阶段和菌落形态相关。菌毛直径约为3至4纳米，形态上与从其他革兰氏阴性菌中分离出的菌毛相似。内部核心结构不明显。纯化菌毛的十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳显示，根据菌落阶段和培养条件，在单个菌株上可观察到多达三种不同的菌毛亚基变体。然而，酶免疫测定和免疫印迹表明这些亚基变体在血清学上相关。用纯化菌毛免疫的小鼠可抵御支气管败血波氏杆菌的强毒腹腔攻击。还发现支气管败血波氏杆菌菌毛在形态、菌毛亚基的分子大小和抗原结构方面与百日咳博德特氏菌菌毛相似。然而，支气管败血波氏杆菌和百日咳博德特氏菌完整菌毛的血清学交叉反应似乎较弱。
