Khakzad Z, Hedayati M T, Mahdian S, Mayahi S
Student Research Committee, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Curr Med Mycol. 2015 Jun;1(2):25-30. doi: 10.18869/acadpub.cmm.1.2.25.
Although molds are regarded as the main fungal allergen sources, evidence indicates that spores of including () can be also found at high concentrations in the environment and may cause as many respiratory allergies as molds. The aim of the present study was to evaluate specific immunoglobulin E (IgE) and immunoglobulin G (IgG) antibodies against via immunoblotting technique in individuals working at mushroom cultivation centers.
In this study, 72 workers involved in the cultivation and harvest of button mushrooms were enrolled. For the analysis of serum IgE and IgG, grown in Sabouraud dextrose broth was harvested and ruptured by liquid nitrogen and glass beads. The obtained sample was centrifuged and the supernatant was collected as "crude extract" (CE). CE was separated via Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The separated proteins were transferred to a nitrocellulose filter and the bands responsive to IgE and IgG were identified by anti-human conjugated antibodies. All participants were screened in terms of total IgE level.
Among 72 workers, 18 (25%) had a total IgE level higher than 188 IU/mL. In SDS-PAGE, the CE of showed 23 different protein bands with a molecular weight range of 13-80 kDa. The sera of 23.6% and 55.5% of participants showed positive response, with specific IgE and IgG antibodies against in the blot, respectively. The bands with molecular weights of 62 and 68 kDa were the most reactive protein components of to specific IgE antibodies. Moreover, bands with molecular weights of 57 and 62 kDa showed the highest reactivity to IgG, respectively. Also, 62 and 68 kDa components were the most reactive bands with both specific IgG and IgE antibodies.
The obtained findings revealed that has different allergens and antigens, which contribute to its potential as an aeroallergen in hypersensitivity-related reactions of the lungs.
尽管霉菌被视为主要的真菌过敏原来源，但有证据表明，包括（）在内的孢子在环境中也可大量存在，且可能引发与霉菌一样多的呼吸道过敏反应。本研究的目的是通过免疫印迹技术评估蘑菇种植中心工作人员针对的特异性免疫球蛋白E（IgE）和免疫球蛋白G（IgG）抗体。
本研究纳入了72名参与双孢蘑菇种植和收获的工人。为分析血清IgE和IgG，将在沙氏葡萄糖肉汤中培养的收获后，用液氮和玻璃珠破碎。所得样品离心后，收集上清液作为“粗提物”（CE）。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离CE。将分离的蛋白质转移到硝酸纤维素滤膜上，并用抗人缀合抗体鉴定对IgE和IgG有反应的条带。所有参与者均进行了总IgE水平筛查。
72名工人中，18名（25%）的总IgE水平高于188 IU/mL。在SDS-PAGE中，的CE显示出23条不同的蛋白条带，分子量范围为13 - 80 kDa。分别有23.6%和55.5%的参与者血清在印迹中显示出针对的特异性IgE和IgG抗体阳性反应。分子量为62和68 kDa的条带是对特异性IgE抗体反应性最强的蛋白成分。此外，分子量为57和62 kDa的条带分别对IgG显示出最高反应性。而且，62和68 kDa成分是对特异性IgG和IgE抗体反应性最强的条带。
所得结果表明，具有不同的过敏原和抗原，这使其在肺部超敏反应相关反应中作为气传过敏原具有潜在作用。
