Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University (KAU), Jeddah, 21589, Saudi Arabia.
Department of Chemistry of Natural and Microbial Products, National Research Centre, Dokki, Giza, 12622, Egypt.
Appl Biochem Biotechnol. 2018 Jan;184(1):323-349. doi: 10.1007/s12010-017-2553-x. Epub 2017 Jul 6.
Eighty-nine cultured Pseudomonas species isolated from the sediment and water samples collected from five industrial Red Sea regions that have been affected by petroleum and industry. Genotypic (exoT, exoS, exoU, exoY, lasA, lasB, rhlA, rhlB, Pf1, PAGI-1, -2, and -3) and phenotypic (DNase, elastase, lipase, protease, siderophore, antibiotic resistance patterns) characteristics were determined. Out of these isolates, nine Pseudomonas isolates were selected as the hyperactive virulence factors producers along with highly resistant pattern against all antibiotics of different classes included in this study. They were subjected to phenotypic and chemotypic characterization as well as molecular identification through 16S rRNA gene amplification and sequencing. The bioactive metabolites of these nine strains were extracted by ethyl acetate followed by evaluating their cytotoxic activity toward liver tissues, kidney tissues, and other biochemical activities in rat. Both EGY6 and EGY8 caused the highest significant reduction in the levels of packed cell volume (PCV), red blood cell count (RBC), and hemoglobin (Hb), which indicate that these Pseudomonas strain metabolites could cause anemia and toxic effects on hematological values in animals that were infected with them. Rats treated with the most toxic extract, EGY8, showed severe histopathological alterations in liver and kidney.
从受石油和工业影响的五个红海工业区域的沉积物和水样中分离出 89 种假单胞菌属物种。对其基因型（exoT、exoS、exoU、exoY、lasA、lasB、rhlA、rhlB、Pf1、PAGI-1、-2 和 -3）和表型（DNase、弹性蛋白酶、脂肪酶、蛋白酶、铁载体、抗生素耐药模式）特征进行了测定。在这些分离物中，选择了 9 个假单胞菌分离物作为高活性毒力因子产生菌，对包括在本研究中的所有不同类别的抗生素均具有高度耐药模式。通过 16S rRNA 基因扩增和测序对它们进行了表型和化感特征以及分子鉴定。用乙酸乙酯提取这 9 株菌的生物活性代谢物，然后评估它们对肝脏组织、肾脏组织和大鼠其他生化活性的细胞毒性。EGY6 和 EGY8 均导致红细胞压积（PCV）、红细胞计数（RBC）和血红蛋白（Hb）水平显著降低，这表明这些假单胞菌株的代谢物可能导致贫血和对感染动物的血液学值产生毒性作用。用最毒提取物 EGY8 处理的大鼠在肝脏和肾脏中表现出严重的组织病理学改变。
