Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt.
Department of Bacteriology, Mycology & Immunology, University of Sadat City, Egypt.
Future Microbiol. 2019 May;14:609-622. doi: 10.2217/fmb-2018-0293. Epub 2019 Apr 18.
This research pioneers the process of obtaining information concerning the distribution and existence of seven genes linked to , three virulence and five quorum sensing separated from 100 camel meat samples using PCR. The Vitek system was used to identify species. Phenotypic antibiotic resistance of 16 antibiotics was tested by disc diffusion. Quantification of pyocyanin, elastase, alkaline protease, biofilm and Vero cell cytotoxicity was also implemented. The total number of species isolated from camel meat was 10/100 identified as 8/10, 2/10. The isolates were multidrug resistant and were resistant to four to eight antibiotics representing four to six classes. The 15 genes exhibited a huge diversity in their association. The results indicated that camel meat is an unpropitious hotbed for species of clinical significance.
本研究采用 PCR 技术，从 100 份骆驼肉样本中分离出与 3 种毒力和 5 种群体感应相关的 7 个基因，开创了获得有关这些基因分布和存在信息的先河。采用 Vitek 系统鉴定 种。采用纸片扩散法检测 16 种抗生素的表型抗生素耐药性。还实施了绿脓菌素、弹性蛋白酶、碱性蛋白酶、生物膜和 Vero 细胞细胞毒性的定量检测。从骆驼肉中分离出的 种总数为 10/100，鉴定为 8/10、2/10。分离株呈多药耐药性，对代表 4 至 6 类的四种至八种抗生素具有耐药性。15 个基因在其关联中表现出巨大的多样性。结果表明，骆驼肉是具有临床意义的 种的不利温床。
