Azimi Somayeh, Kafil Hossein Samadi, Baghi Hossein Bannazadeh, Shokrian Saeed, Najaf Khadijeh, Asgharzadeh Mohammad, Yousefi Mehdi, Shahrivar Firooz, Aghazadeh Mohammad
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
GMS Hyg Infect Control. 2016 Feb 22;11:Doc04. doi: 10.3205/dgkh000264. eCollection 2016.
Pseudomonas aeruginosa, as Gram-negative rod bacilli, has an important role in human infection. In the present study we aimed to investigate the presence of exo genes and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran.
160 isolates of P. aeruginosa were collected and identified by biochemical tests and were characterized for antibiotic resistance. Biofilm production was evaluated by microtiter plate assay and the presence of exo genes was evaluated by allele-specific PCR (polymerase chain reaction). Chi-square test was used for statistical analysis.
The most effective antibiotics against isolates were colistin and polymyxin B. 87% of the isolates were biofilm producers of which 69% were strongly biofilm producers. 55% of the isolates carried exoY, 52% of the isolates carried exoU, and 26.3% and 5% carried exoS and exoT, respectively.
Our findings showed different distribution of exo genes in clinical isolates of P. aeruginosa in Northwest Iran. ExoS and exoU were more prevalent in non-biofilm producers and exoY was more prevalent in biofilm producer isolates. These results might indicate the importance of exoY in biofilm production of Pseudomonas aeruginosa.
铜绿假单胞菌作为革兰氏阴性杆菌，在人类感染中起重要作用。在本研究中，我们旨在调查伊朗西北部铜绿假单胞菌分离株中外毒素基因的存在情况及生物膜形成情况。
收集160株铜绿假单胞菌，通过生化试验进行鉴定，并对其抗生素耐药性进行表征。通过微量滴定板法评估生物膜形成情况，通过等位基因特异性聚合酶链反应（PCR）评估外毒素基因的存在情况。采用卡方检验进行统计分析。
对分离株最有效的抗生素是黏菌素和多黏菌素B。87%的分离株是生物膜产生菌，其中69%是强生物膜产生菌。55%的分离株携带exoY，52%的分离株携带exoU，分别有26.3%和5%的分离株携带exoS和exoT。
我们的研究结果显示伊朗西北部铜绿假单胞菌临床分离株中外毒素基因的分布不同。ExoS和exoU在非生物膜产生菌中更为普遍，而exoY在生物膜产生菌分离株中更为普遍。这些结果可能表明exoY在铜绿假单胞菌生物膜形成中的重要性。
