Yang Woong-Suk, Yang Seung-Hoon, Lee Jae-Yong, Jang Seong-Ho, Kim Cheorl-Ho, Hwnag Cher-Won
School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu. Korea.
Systems Biotechnology Research Center, Korea Institute of Science and Technology (KIST), Gangneung. Korea.
Curr Pharm Biotechnol. 2017;18(7):552-559. doi: 10.2174/1389201018666170705165337.
Persicaria is a genus of flowering plants generally used for traditional medicine and nutritional supplements in tropical and subtropical East Asian countries. Previous studies have shown that Persicaria extracts alleviate lipid peroxidation, hypertension, and inflammation.
We investigated the anti-oxidative and anti-microbial effects of ethanol extracts of Persicaria nepalensis (Meisn.) Miyabe, and isolated and identified an active compound, MPN-1-1 from the ethanol extracts.
Anti-oxidative values, as indicated by the Oxygen Radical Absorbance Capacity (ORAC) assay, were enhanced by treatment with Persicaria nepalensis (Meisn.) Miyabe ethanol extracts, and bacterial growth was inhibited. The active compound (MPN-1-1), which was further isolated and purified from a Persicaria nepalensis (Meisn.) Miyabe ethanol extract by medium pressure liquid chromatography (MPLC), also had strong anti-oxidative and anti-microbial activity. 1H-NMR spectroscopy identified MPN-1-1 as a 1-ethenyl-4,8-dimethoxy-9H-pyrido(3,4-β) indole compound, which is an alkaloid.
Our results provide evidence that Persicaria nepalensis (Meisn.) Miyabe extract has strong physiological activity without any toxic effects, and furthermore, MPN-1-1 can be potentially utilized as a natural dietary supplement as well as an anti-oxidant.
蓼属是一类开花植物，在东亚热带和亚热带国家通常用于传统医学和营养补充剂。先前的研究表明，蓼属植物提取物可减轻脂质过氧化、高血压和炎症。
我们研究了尼泊尔蓼（Meisn.）Miyabe乙醇提取物的抗氧化和抗菌作用，并从乙醇提取物中分离鉴定了一种活性化合物MPN-1-1。
通过氧自由基吸收能力（ORAC）测定表明，尼泊尔蓼（Meisn.）Miyabe乙醇提取物处理可提高抗氧化值，并抑制细菌生长。通过中压液相色谱（MPLC）从尼泊尔蓼（Meisn.）Miyabe乙醇提取物中进一步分离纯化得到的活性化合物（MPN-1-1）也具有很强的抗氧化和抗菌活性。1H-NMR光谱鉴定MPN-1-1为1-乙烯基-4,8-二甲氧基-9H-吡啶并（3,4-β）吲哚化合物，即一种生物碱。
我们的结果提供了证据，表明尼泊尔蓼（Meisn.）Miyabe提取物具有很强的生理活性且无任何毒性作用，此外，MPN-1-1可潜在地用作天然膳食补充剂以及抗氧化剂。
