Jovanović Marina, Morić Ivana, Nikolić Biljana, Pavić Aleksandar, Svirčev Emilija, Šenerović Lidija, Mitić-Ćulafić Dragana
Faculty of Biology, University of Belgrade, Studentski trg 16, 11158 Belgrade, Serbia.
Institute of General and Physical Chemistry, Studentski trg 12/V, 11158 Belgrade, Serbia.
Molecules. 2020 Apr 15;25(8):1811. doi: 10.3390/molecules25081811.
Many traditional remedies represent potential candidates for integration with modern medical practice, but credible data on their activities are often scarce. For the first time, the anti-virulence potential and the safety for human use of the ethanol extracts of two medicinal plants, (PEM) and (BIO), have been addressed. Ethanol extracts of both plants exhibited anti-virulence activity against the medically important opportunistic pathogen . At the subinhibitory concentration of 50 µg/mL, the extracts demonstrated a maximal inhibitory effect (approx. 50%) against biofilm formation, the highest reduction of pyocyanin production (47% for PEM and 59% for BIO) and completely halted the swarming motility of . Both extracts demonstrated better anti-quorum sensing and antibiofilm activities, and a better ability to interfere with LasR receptor, than the tested dominant extracts' constituents. The bioactive concentrations of the extracts were not toxic in the zebrafish model system. This study represents an initial step towards the integration of and for use in the treatment of infections.
许多传统疗法有望与现代医学实践相结合，但关于其活性的可靠数据往往匮乏。首次探讨了两种药用植物（PEM）和（BIO）乙醇提取物的抗毒力潜力及对人体使用的安全性。两种植物的乙醇提取物均对医学上重要的机会性病原体表现出抗毒力活性。在50 µg/mL的亚抑制浓度下，提取物对生物膜形成表现出最大抑制作用（约50%），对绿脓菌素产生的降低幅度最大（PEM为47%，BIO为59%），并完全阻止了该病原体的群游运动。与测试的主要提取物成分相比，两种提取物均表现出更好的群体感应抑制和抗生物膜活性，以及更好的干扰LasR受体的能力。提取物的生物活性浓度在斑马鱼模型系统中无毒。本研究是将PEM和BIO用于治疗该病原体感染的整合迈出的第一步。
