Looman Kirsten I M, Jansen Michelle A E, Voortman Trudy, van den Heuvel Diana, Jaddoe Vincent W V, Franco Oscar H, van Zelm Menno C, Moll Henriëtte A
Generation R Study Group, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
Department of Pediatrics, Sophia Children's Hospital, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
Pediatr Allergy Immunol. 2017 Sep;28(6):579-587. doi: 10.1111/pai.12754. Epub 2017 Aug 7.
Previous studies have demonstrated that vitamin D affects T-cell function and maturation via the vitamin D receptor. However, no studies in children have been performed on this topic. Because most of the T-cell memory is formed in the first 5 years of life, we aimed to determine the association between serum 25-hydroxyvitamin D (25(OH)D) levels and numbers of circulatory naive, central memory (Tcm), and effector memory (Tem) T lymphocytes in a large population of healthy children.
Among 3189 children participating in a population-based prospective cohort, we measured 25(OH)D levels and performed detailed immunophenotyping of naive and memory T lymphocytes at a median age of 6.0 years (95% range 5.7-7.9). Detailed lymphocyte subsets were available in 986 children. Multivariable linear regression analyses were performed to determine the association between 25(OH)D and the maturation of T lymphocytes in children adjusted for cord blood 25(OH)D levels, herpes seropositivity, sociodemographic and lifestyle confounders. Furthermore, multivariable logistic regression analyses were performed to determine associations between 25(OH)D and childhood infections.
Higher 25(OH)D levels were associated with higher numbers of Tem lymphocytes. Every 10 nmol/L higher 25(OH)D was associated with 2.20% (95% CI 0.54-3.89; P=.009) higher CD4TemRA, 1.50% (95% CI 0.38-2.62; P=.008) higher CD4TemRO, and 1.82% (95% CI 0.11-3.56; P=.037) higher CD8TemRA cell numbers. Generally, stronger associations were observed among boys. 25(OH)D levels were not significantly associated with naive, Tcm cell numbers, herpes seropositivity, or URTIs.
Our results suggest that vitamin D enhances cellular immunity in young children.
先前的研究表明，维生素D通过维生素D受体影响T细胞功能和成熟。然而，尚未针对该主题对儿童进行研究。由于大多数T细胞记忆在生命的前5年形成，我们旨在确定大量健康儿童血清25-羟基维生素D（25(OH)D）水平与循环中幼稚、中枢记忆（Tcm）和效应记忆（Tem）T淋巴细胞数量之间的关联。
在参与基于人群的前瞻性队列研究的3189名儿童中，我们测量了25(OH)D水平，并在中位年龄6.0岁（95%范围5.7 - 7.9）时对幼稚和记忆T淋巴细胞进行了详细的免疫表型分析。986名儿童有详细的淋巴细胞亚群数据。进行多变量线性回归分析以确定在根据脐血25(OH)D水平、疱疹血清阳性、社会人口统计学和生活方式混杂因素进行调整后，25(OH)D与儿童T淋巴细胞成熟之间的关联。此外，进行多变量逻辑回归分析以确定25(OH)D与儿童感染之间的关联。
较高的25(OH)D水平与较多的Tem淋巴细胞数量相关。25(OH)D每升高10 nmol/L，CD4TemRA细胞数量增加2.20%（95%CI 0.54 - 3.89；P = 0.009），CD4TemRO细胞数量增加1.50%（95%CI 0.38 - 2.62；P = 0.008），CD8TemRA细胞数量增加1.82%（95%CI 0.11 - 3.56；P = 0.037）。一般来说，男孩中的关联更强。25(OH)D水平与幼稚、Tcm细胞数量、疱疹血清阳性或上呼吸道感染无显著关联。
我们的结果表明维生素D可增强幼儿的细胞免疫。
