樟芝复方 CCM111 通过 STAT3 和 NF-κB 通路调节免疫相关活性。
CCM111, the water extract of Antrodia cinnamomea, regulates immune-related activity through STAT3 and NF-κB pathways.
机构信息
Department of Biomedical Sciences and Engineering; Institute of Systems Biology and Bioinformatics, National Central University, Taoyuan, Taiwan.
Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.
出版信息
Sci Rep. 2017 Jul 7;7(1):4862. doi: 10.1038/s41598-017-05072-y.
Antrodia cinnamomea (AC) exhibits many bioactivities, including anti-inflammatory, anti-cancer, and hepatoprotection activities. Many researchers have studied the functions of the components or fractions of AC, but the functions of the original extractions of AC have not been studied. In addition, the detailed relationship between AC and immune-related signaling pathways is unclear. In this study, we screened the effects of CCM111, which is the extract of AC, on seven immune-related signaling pathways and further investigated whether CCM111 can influence inflammation. Interestingly, our results showed that CCM111 significantly inhibited the IL-6-stimulated STAT3 pathway and the LPS-stimulated NF-κB pathway in macrophages. CCM111 also decreased the phosphorylation of STAT3, Tyk2 and the nuclear translocation of p65. Moreover, CCM111 and F4, a fraction of CCM111, down-regulated nitric oxide (NO) production, the protein levels of iNOS and COX-2, and inflammatory cytokines in macrophage cells. Therefore, our study suggested that CCM111 has the potential to be developed as an effective anti-inflammatory agent.
樟芝（Antrodia cinnamomea，AC）具有多种生物活性，包括抗炎、抗癌和保肝活性。许多研究人员研究了 AC 的成分或馏分的功能，但尚未研究 AC 的原始提取物的功能。此外，AC 与免疫相关信号通路之间的详细关系尚不清楚。在这项研究中，我们筛选了 CCM111（AC 的提取物）对七种免疫相关信号通路的影响，并进一步研究了 CCM111 是否能影响炎症。有趣的是，我们的结果表明，CCM111 可显著抑制巨噬细胞中 IL-6 刺激的 STAT3 通路和 LPS 刺激的 NF-κB 通路。CCM111 还降低了 STAT3、Tyk2 的磷酸化和 p65 的核转位。此外，CCM111 和 F4（CCM111 的馏分之一）降低了巨噬细胞中一氧化氮（NO）的产生、iNOS 和 COX-2 的蛋白水平以及炎症细胞因子的水平。因此，我们的研究表明，CCM111 有可能被开发为一种有效的抗炎剂。
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