脆弱拟杆菌的氯霉素乙酰转移酶
Chloramphenicol acetyltransferase of Bacteroides fragilis.
作者信息
Britz M L, Wilkinson R G
出版信息
Antimicrob Agents Chemother. 1978 Jul;14(1):105-11. doi: 10.1128/AAC.14.1.105.
Abstract
Chloramphenicol-resistant strains of Bacteroides fragilis (minimum inhibitory concentration, 12.5 mug/ml) were isolated from a stool specimen which contained multiply resistant Escherichia coli. The enzyme responsible for resistance, chloramphenicol acetyltransferase, was produced constitutively by these strains; the specific activity was 10-fold lower than that of the E. coli enzymes. Similar activity was not detected in susceptible B. fragilis strains, nor could it be induced by growth in the presence of chloramphenicol or by mutagenesis. The enzyme had a pH optimum of 7.8 and a molecular weight of approximately 89,000. The K(m) for chloramphenicol was 5.2 muM, and the enzyme was sensitive to inhibition by 5,5'-dithiobis-2-nitrobenzoic acid. The enzyme produced by an E. coli strain isolated from the same specimen had a similar K(m) and sensitivity to 5,5'-dithiobis-2-nitrobenzoic acid.
摘要
从一份含有多重耐药性大肠杆菌的粪便标本中分离出了脆弱拟杆菌的耐氯霉素菌株（最低抑菌浓度为12.5μg/ml）。这些菌株组成型产生负责耐药性的酶——氯霉素乙酰转移酶；其比活性比大肠杆菌的酶低10倍。在敏感的脆弱拟杆菌菌株中未检测到类似活性，在氯霉素存在下生长或诱变也不能诱导产生该活性。该酶的最适pH为7.8，分子量约为89,000。氯霉素的米氏常数（K(m)）为5.2μM，该酶对5,5'-二硫代双-2-硝基苯甲酸的抑制敏感。从同一份标本中分离出的一株大肠杆菌产生的酶具有相似的米氏常数和对5,5'-二硫代双-2-硝基苯甲酸的敏感性。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The enzymatic acetylation of chloramphenicol by extracts of R factor-resistant Escherichia coli.R因子抗性大肠杆菌提取物对氯霉素的酶促乙酰化作用。
J Biol Chem. 1967 Feb 25;242(4):687-93.
3
Characterization of chloramphenicol acetyltransferase from chloramphenicol-resistant Staphylococcus aureus.耐氯霉素金黄色葡萄球菌中氯霉素乙酰转移酶的特性分析
J Bacteriol. 1968 Jan;95(1):28-36. doi: 10.1128/jb.95.1.28-36.1968.
4
Bacteriostatic and bactericidal activities of various antibiotics against Bacteroides fragilis.多种抗生素对脆弱拟杆菌的抑菌和杀菌活性
Antimicrob Agents Chemother. 1973 Feb;3(2):152-6. doi: 10.1128/AAC.3.2.152.
5
Antibiotic susceptibility testing of Bacteroides.拟杆菌属的抗生素敏感性测试。
Antimicrob Agents Chemother. 1972 Aug;2(2):57-60. doi: 10.1128/AAC.2.2.57.
6
Anaerobic bacteriology. Isolation and identification of Bacteroides and Fusobacterium from human sources.厌氧细菌学。从人体样本中分离和鉴定拟杆菌属和梭杆菌属。
Aust J Exp Biol Med Sci. 1974 Dec;52(6):897-908.
7
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8
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9
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