Laopichienpong Nararat, Tawichasri Panupong, Chanhome Lawan, Phatcharakullawarawat Rattanin, Singchat Worapong, Kantachumpoo Attachai, Muangmai Narongrit, Suntrarachun Sunutcha, Matsubara Kazumi, Peyachoknagul Surin, Srikulnath Kornsorn
Laboratory of Animal Cytogenetics and Comparative Genomics (ACCG) Department of Genetics Faculty of Science Kasetsart University Bangkok Thailand.
Animal Breeding and Genetics Consortium of Kasetsart University (ABG - KU) Bangkok Thailand.
Ecol Evol. 2017 May 22;7(13):4661-4669. doi: 10.1002/ece3.3057. eCollection 2017 Jul.
Sex identification provides important information for ecological and evolutionary studies, as well as benefiting snake conservation management. Traditional methods such as cloacal probing or cloacal popping are counterproductive for sex identification concerning very small species, resulting in difficulties in the management of their breeding programs. In this study, the nucleotide sequences of gametologous genes ( and genes) were used for the development of molecular sexing markers in caenophidian snakes. Two candidate markers were developed with the two primer sets, and successfully amplified by a single band on the agarose gel in male (ZZ) and two bands, differing in fragment sizes, in female (ZW) of 16 caenophidian snakes for and 12 caenophidian snakes for . Another candidate marker was developed with the primer set to amplify the specific sequence for W homolog, and the PCR products were successfully obtained in a female-specific 250-bp DNA bands. The three candidate PCR sexing markers provide a simple sex identification method based on the amplification of gametologous genes, and they can be used to facilitate effective caenophidian snake conservation and management programs.
性别鉴定为生态和进化研究提供了重要信息，同时也有利于蛇类的保护管理。传统的方法，如泄殖腔探针检查或泄殖腔外翻，对于非常小的蛇类物种进行性别鉴定时会适得其反，导致其繁殖计划管理困难。在本研究中，利用配子同源基因（ 和 基因）的核苷酸序列开发了新蛇亚目蛇类的分子性别鉴定标记。用两组引物开发了两个候选标记，并在16种新蛇亚目蛇类的雄性（ZZ）中通过琼脂糖凝胶上的单一条带成功扩增，在雌性（ZW）中通过两条大小不同的条带成功扩增， 基因鉴定了16种新蛇亚目蛇类， 基因鉴定了12种新蛇亚目蛇类。用引物组开发了另一个候选标记，以扩增W同源物的特定序列，PCR产物成功获得了雌性特异性的250 bp DNA条带。这三个候选PCR性别鉴定标记基于配子同源基因的扩增提供了一种简单的性别鉴定方法，可用于促进有效的新蛇亚目蛇类保护和管理计划。
