Changizi Vahid, Bahrami Mona, Esfahani Mahbod, Shetab-Boushehri Seyed V
Department of Technology of Radiology & Radiotherapy, Allied Medical Sciences School, Tehran, Iran.
Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran.
Sultan Qaboos Univ Med J. 2017 May;17(2):e162-e167. doi: 10.18295/squmj.2016.17.02.005. Epub 2017 Jun 20.
Ionising radiation has deleterious effects on human cells. N-acetylcysteine (NAC) and cysteine, the active metabolite of NAC, are well-known radioprotective agents. Recently, a serine-magnesium sulfate combination was proposed as an antidote for organophosphate toxicity. This study aimed to investigate the use of a serine-magnesium sulfate mixture in the prevention of γ-radiation-induced DNA damage in human lymphocytes as compared to NAC and cysteine.
This study was carried out at the Iran University of Medical Sciences, Tehran, Iran, between April and September 2016. Citrated blood samples of 7 mL each were taken from 22 healthy subjects. Each sample was divided into 1 mL , with the first acting as the control while the second was exposed to 2 Gy of γ-radiation at a dose rate of 102.7 cGy/minute. The remaining were separately incubated with 600 μM concentrations each of serine, magnesium sulfate, serine-magnesium sulfate, NAC and cysteine before being exposed to 2 Gy of γ-radiation. Lymphocytes were isolated using a separation medium and methyl-thiazole-tetrazolium and comet assays were used to evaluate cell viability and DNA damage, respectively.
The serine-magnesium sulfate mixture significantly increased lymphocyte viability and reduced DNA damage in comparison to serine, magnesium sulfate, NAC or cysteine alone ( <0.01 each).
The findings of the present study support the use of a serine-magnesium sulfate mixture as a new, non-toxic, potent and efficient radioprotective agent.
电离辐射对人体细胞具有有害影响。N-乙酰半胱氨酸（NAC）及其活性代谢产物半胱氨酸是众所周知的辐射防护剂。最近，有人提出丝氨酸 - 硫酸镁组合可作为有机磷中毒的解毒剂。本研究旨在调查与NAC和半胱氨酸相比，丝氨酸 - 硫酸镁混合物在预防γ射线诱导的人类淋巴细胞DNA损伤中的应用。
本研究于2016年4月至9月在伊朗德黑兰的伊朗医科大学进行。从22名健康受试者中采集每份7 mL的枸橼酸盐血样。每个样本分为1 mL，第一个作为对照，第二个以102.7 cGy/分钟的剂量率接受2 Gy的γ射线照射。其余样本在接受2 Gy的γ射线照射之前，分别与600 μM浓度的丝氨酸、硫酸镁、丝氨酸 - 硫酸镁、NAC和半胱氨酸孵育。使用分离介质分离淋巴细胞，并分别使用甲基噻唑四氮唑和彗星试验评估细胞活力和DNA损伤。
与单独使用丝氨酸、硫酸镁、NAC或半胱氨酸相比，丝氨酸 - 硫酸镁混合物显著提高了淋巴细胞活力并减少了DNA损伤（每项P<0.01）。
本研究结果支持将丝氨酸 - 硫酸镁混合物用作一种新型、无毒、高效且有效的辐射防护剂。
