Si Hua-Yang, Chen Nai-Fu, Chen Nai-Dong, Huang Cheng, Li Jun, Wang Hui
a College of Biotechnology and Pharmaceutical Engineering , West Anhui University , Lu'an City , China.
b West Anhui Biotechnology Research Center of Natural Medicine and Traditional Chinese Medicine , West Anhui University , Lu'an City , China.
Nat Prod Res. 2018 Feb;32(3):252-260. doi: 10.1080/14786419.2017.1350670. Epub 2017 Jul 10.
A water-soluble polysaccharide TC-DHPA4 with a molecular weight of 8.0 × 10 Da was isolated from tissue-cultured Dendrobium huoshanense by anion exchange and gel permeation chromatography. Monosaccharide analysis revealed that the homogeneous polysaccharide was made up of rhamnose, arabinose, mannose, glucose, galactose and glucuronic acid with a molar ratio of 1.28:1:1.67:4.71:10.43:1.42. The sugar residue sequence analysis based on the GC-MS files and NMR spectra indicated that the backbone of TC-DHPA4 consisted of the repeated units:→6)-β-Galp-(1→6)-β-Galp-(1→4)-β-GlcpA-(1→6)-β-Glcp-(1→6)-β-Glcp-(→. The sugar residue sequences β-Glcp-(1→)-α-Rhap-(1→3)-β-Galp-(1→, β-Glcp-(1→4)-α-Rhap-(1→3)-β-Galp-(1→, β-Galp-(1→6)-β-Manp-(1→3)-β-Galp-(1→, and α-l-Araf-(1→2)-β-Manp-(1→3)-β-Galp-(1→ were identified as the branches attached to the C-3 position of (1→6)-linked galactose in the backbone.
通过阴离子交换和凝胶渗透色谱法从组培霍山石斛中分离得到一种分子量为8.0×10 Da的水溶性多糖TC-DHPA4。单糖分析表明，该均一多糖由鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖和葡萄糖醛酸组成，摩尔比为1.28:1:1.67:4.71:10.43:1.42。基于气相色谱-质谱联用（GC-MS）文件和核磁共振（NMR）光谱的糖残基序列分析表明，TC-DHPA4的主链由重复单元→6)-β-半乳糖基-(1→6)-β-半乳糖基-(1→4)-β-葡萄糖醛酸基-(1→6)-β-葡萄糖基-(1→6)-β-葡萄糖基-(→组成。糖残基序列β-葡萄糖基-(1→)-α-鼠李糖基-(1→3)-β-半乳糖基-(1→、β-葡萄糖基-(1→4)-α-鼠李糖基-(1→3)-β-半乳糖基-(1→、β-半乳糖基-(1→6)-β-甘露糖基-(1→3)-β-半乳糖基-(1→和α-L-阿拉伯糖基-(1→2)-β-甘露糖基-(1→3)-β-半乳糖基-(1→被鉴定为连接在主链中(1→6)-连接的半乳糖C-3位上的分支。
