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亚临界水提取的一种新型多糖的结构与生物活性

Structure and Bioactivities of a Novel Polysaccharide Extracted From by Subcritical Water.

作者信息

Wang Li, Mao Yi-Gui, Zeng Xiang, Liu Na, Niu Chao-Fei, Li Xin-Xin, Ma Bing-Ji, Guo Lan-Ping, Yang Xiao-Long

机构信息

The Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei Province, School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China.

Department of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou, China.

出版信息

Front Nutr. 2022 Apr 26;9:877871. doi: 10.3389/fnut.2022.877871. eCollection 2022.

DOI:10.3389/fnut.2022.877871
PMID:35558752
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9087634/
Abstract

In this study, the polysaccharide was extracted by subcritical water from . A novel polysaccharide (DHPs-1) was obtained through several purification steps and its structure and bioactivity were investigated. Structural analysis indicated that the weight-average molecular weight of DHPs-1 was 5.0 × 10 Da and it was mainly composed of glucose (65.04%), mannose (14.23%), galactose (8.17%), galacturonic acid (6.41%), rhamnose (2.34%), and xylose (1.25%). 1,4-Glc, and 1,4,6-Gal were existed in the backbone of DHPs-1. The residues of 1,3,4-Gal, 1,4-Man, 1,4-Gal, and 1,3,4,6-Gal could be in the backbone or the side chains with the non-reducing terminal of α-Man. Bioactivity tests indicated that DHPs-1 had immunomodulatory activity in that it significantly enhanced transcript levels of cytokines [Tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), Interleukin-1β (IL-1β), and Interleukin-10 (IL-10)]. DPPH and hydroxyl radical scavenging tests showed that it had good antioxidant activity. These results reveal that DHPs-1 could be developed as a safe immunomodulatory agent and antioxidant for pharmacological or functional food applications.

摘要

在本研究中，采用亚临界水从……中提取多糖。通过几步纯化步骤获得了一种新型多糖（DHPs-1），并对其结构和生物活性进行了研究。结构分析表明，DHPs-1的重均分子量为5.0×10 Da，其主要由葡萄糖（65.04%）、甘露糖（14.23%）、半乳糖（8.17%）、半乳糖醛酸（6.41%）、鼠李糖（2.34%）和木糖（1.25%）组成。1,4-葡萄糖和1,4,6-半乳糖存在于DHPs-1的主链中。1,3,4-半乳糖、1,4-甘露糖、1,4-半乳糖和1,3,4,6-半乳糖的残基可能存在于主链或侧链中，且具有α-甘露糖的非还原末端。生物活性测试表明，DHPs-1具有免疫调节活性，因为它能显著提高细胞因子[肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）和白细胞介素-10（IL-10）]的转录水平。DPPH和羟基自由基清除试验表明它具有良好的抗氧化活性。这些结果表明，DHPs-1可被开发为一种安全的免疫调节剂和抗氧化剂，用于药理学或功能性食品应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/4aaf4b2506fa/fnut-09-877871-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/4115750f13da/fnut-09-877871-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/ed0c1221e1e4/fnut-09-877871-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/53e15cbe461a/fnut-09-877871-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/4b3b990877d2/fnut-09-877871-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/76517e73d472/fnut-09-877871-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/8c05345e3ab9/fnut-09-877871-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/4aaf4b2506fa/fnut-09-877871-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/4115750f13da/fnut-09-877871-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/ed0c1221e1e4/fnut-09-877871-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/53e15cbe461a/fnut-09-877871-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/4b3b990877d2/fnut-09-877871-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/76517e73d472/fnut-09-877871-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/8c05345e3ab9/fnut-09-877871-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e871/9087634/4aaf4b2506fa/fnut-09-877871-g0007.jpg

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