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杂合性羊瘙痒症阳性山羊中，222K PrP变体在抗蛋白酶部分PrPres中的比例较低。

Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats.

作者信息

Mazza Maria, Guglielmetti Chiara, Ingravalle Francesco, Brusadore Sonia, Langeveld Jan P M, Ekateriniadou Loukia V, Andréoletti Olivier, Casalone Cristina, Acutis Pier Luigi

机构信息

Italian Reference Centre for TSEs, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Turin, Italy.

Biostatistic, Epidemiology and Risk Analysis Unit, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Turin, Italy.

出版信息

J Gen Virol. 2017 Jul;98(7):1963-1967. doi: 10.1099/jgv.0.000843. Epub 2017 Jul 13.

DOI:10.1099/jgv.0.000843
PMID:28691895
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5656779/
Abstract

The presence of lysine (K) at codon 222 has been associated with resistance to classical scrapie in goats, but few scrapie cases have been identified in 222Q/K animals. To investigate the contribution of the 222K variant to PrPres formation in natural and experimental Q/K scrapie cases, we applied an immunoblotting method based on the use of two different monoclonal antibodies, F99/97.6.1 and SAF84, chosen for their different affinities to 222K and 222Q PrP variants. Our finding that PrPres seems to be formed nearly totally by the 222Q variant provides evidence that the 222K PrP variant confers resistance to conversion to PrPres formation and reinforces the view that this mutation has a protective role against classical scrapie in goats.

摘要

密码子222处赖氨酸（K）的存在与山羊对经典痒病的抗性有关，但在222Q/K动物中仅发现了少数痒病病例。为了研究222K变体在自然和实验性Q/K痒病病例中对PrPres形成的作用，我们应用了一种免疫印迹方法，该方法基于使用两种不同的单克隆抗体F99/97.6.1和SAF84，选择这两种抗体是因为它们对222K和222Q PrP变体具有不同的亲和力。我们的研究结果表明，PrPres似乎几乎完全由222Q变体形成，这一发现证明222K PrP变体赋予了对转化为PrPres形成的抗性，并强化了这种突变对山羊经典痒病具有保护作用的观点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba21/5656779/cc6d675ae542/jgv-98-1963-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba21/5656779/0012d78b8ca3/jgv-98-1963-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba21/5656779/cc6d675ae542/jgv-98-1963-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba21/5656779/0012d78b8ca3/jgv-98-1963-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba21/5656779/cc6d675ae542/jgv-98-1963-g002.jpg

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引用本文的文献

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Animals (Basel). 2021 Aug 8;11(8):2340. doi: 10.3390/ani11082340.
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Large-scale survey of prion protein genetic variability in scrapie disease-free goats from the United States.美国无瘙痒病绵羊朊病毒蛋白遗传变异的大规模调查。
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本文引用的文献

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Comparative Susceptibility of Sheep of Different Origins, Breeds and PRNP Genotypes to Challenge with Bovine Spongiform Encephalopathy and Scrapie.不同来源、品种和PRNP基因型绵羊对牛海绵状脑病和羊瘙痒病攻击的易感性比较
PLoS One. 2015 Nov 20;10(11):e0143251. doi: 10.1371/journal.pone.0143251. eCollection 2015.
2
Role of the goat K222-PrP(C) polymorphic variant in prion infection resistance.山羊K222-PrP(C)多态变体在朊病毒感染抗性中的作用。
J Virol. 2014 Mar;88(5):2670-6. doi: 10.1128/JVI.02074-13. Epub 2013 Dec 18.
3
Genetic resistance to scrapie infection in experimentally challenged goats.
山羊朊病毒蛋白 PRNP 多态性 N146S 和 Q222K 与 PrP 的蛋白水解切割有关。
Genet Sel Evol. 2021 Jun 19;53(1):52. doi: 10.1186/s12711-021-00646-x.
4
Genetic profile of scrapie codons 146, 211 and 222 in the PRNP gene locus in three breeds of dairy goats.PRNP 基因座中 146、211 和 222 密码子的羊瘙痒病遗传特征在三个奶山羊品种中的研究。
PLoS One. 2018 Jun 7;13(6):e0198819. doi: 10.1371/journal.pone.0198819. eCollection 2018.
实验性感染山羊对瘙痒病感染的遗传抗性
J Virol. 2014 Mar;88(5):2406-13. doi: 10.1128/JVI.02872-13. Epub 2013 Nov 27.
4
PrP-associated resistance to scrapie in five highly infected goat herds.五群高度感染的山羊群中朊病毒相关的抗瘙痒病能力。
J Gen Virol. 2013 Jan;94(Pt 1):241-245. doi: 10.1099/vir.0.047225-0. Epub 2012 Oct 24.
5
Lysine at position 222 of the goat prion protein inhibits the binding of monoclonal antibody F99/97.6.1.山羊朊病毒蛋白第222位的赖氨酸抑制单克隆抗体F99/97.6.1的结合。
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6
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Gene. 2012 Jun 10;501(1):49-51. doi: 10.1016/j.gene.2012.03.068. Epub 2012 Apr 4.
7
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