Thitilertdecha Premrutai, Khowawisetsut Ladawan, Ammaranond Palanee, Poungpairoj Poonsin, Tantithavorn Varangkana, Onlamoon Nattawat
Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Dis Markers. 2017;2017:5729639. doi: 10.1155/2017/5729639. Epub 2017 Jun 12.
Antiretroviral therapy (ART) is generally prescribed to patients with human immunodeficiency virus (HIV) infection with vaccination introduced to prevent disease complications. However, little is known about the influence of immunization on T cell subsets' distribution during the course of infection. This study aims to identify the impact of viral replication and immunization on naïve, effector, effector memory, and central memory T cell subpopulations in ART-treated HIV-infected children. Fifty patients were recruited and injected intramuscularly with influenza A (H1N1) 2009 vaccine on the day of enrollment (day 0) and day 28. Blood samples were collected for pre- and postvaccination on days 0 and 56 for analyzing T cell phenotypes by flow cytometry. Phenotypes of all T cell subsets remained the same after vaccination, except for a reduction in effector CD8 T cells. Moreover, T cell subsets from patients with controllable viral load showed similar patterns to those with virological failure. Absolute CD4 count was also found to have a positive relationship with naïve CD4 and CD8 T cells. In conclusion, vaccination and viral replication have a little effect on the distribution of T cell subpopulations. The CD4 count can be used for prediction of naïve T cell level in HIV-infected patients responding to ART.
抗逆转录病毒疗法（ART）通常会开给感染人类免疫缺陷病毒（HIV）的患者，并引入疫苗接种以预防疾病并发症。然而，关于免疫接种在感染过程中对T细胞亚群分布的影响，人们知之甚少。本研究旨在确定病毒复制和免疫接种对接受抗逆转录病毒治疗的HIV感染儿童中初始、效应、效应记忆和中枢记忆T细胞亚群的影响。招募了50名患者，并在入组当天（第0天）和第28天肌肉注射2009甲型（H1N1）流感疫苗。在第0天和第56天采集接种疫苗前后的血样，通过流式细胞术分析T细胞表型。除效应性CD8 T细胞减少外，接种疫苗后所有T细胞亚群的表型均保持不变。此外，病毒载量可控的患者的T细胞亚群与病毒学失败患者的T细胞亚群表现出相似的模式。还发现绝对CD4计数与初始CD4和CD8 T细胞呈正相关。总之，疫苗接种和病毒复制对T细胞亚群的分布影响不大。CD4计数可用于预测接受抗逆转录病毒治疗的HIV感染患者的初始T细胞水平。
