Boström P J, Ravanti L, Reunanen N, Aaltonen V, Söderström K O, Kähäri V M, Laato M
Department of Surgery, Turku University Central Hospital, Finland.
Int J Cancer. 2000 Nov 1;88(3):417-23.
Expression of collagenase-3 [matrix metalloproteinase-13 (MMP-13)] has been previously demonstrated in squamous-cell carcinomas of both the head and neck and the vulva, cutaneous basal-cell carcinomas, chondrosarcomas and melanomas. Using in situ hybridization, MMP-13 mRNA expression was detected in 13 of 23 (52%) urinary bladder transitional-cell carcinomas (TCCs). Expression was restricted to cells in the invading edges of tumors. No expression of MMP-13 mRNA could be detected in normal urothelium. As detected by immunohistochemistry, MMP-13 protein showed an expression pattern similar to that of MMP-13 mRNA. Expression of MMP-13 mRNA and protein was also detected in 2 bladder carcinoma cell lines (RT4 and T24). In these cell lines, TNF-alpha potently induced MMP-13 mRNA expression. Retinoids and a selective p38 inhibitor, SB203580, potently inhibited MMP-13 mRNA expression. Our results demonstrate MMP-13 expression in human urinary bladder carcinoma cells in vivo and in vitro and suggest that MMP-13 may serve as a marker for transformation and invasion in urinary bladder TCCs.
先前已证实在头颈部和外阴的鳞状细胞癌、皮肤基底细胞癌、软骨肉瘤和黑色素瘤中存在胶原酶-3[基质金属蛋白酶-13（MMP-13）]的表达。采用原位杂交技术，在23例膀胱移行细胞癌（TCC）中的13例（52%）检测到MMP-13 mRNA表达。表达局限于肿瘤浸润边缘的细胞。在正常尿路上皮中未检测到MMP-13 mRNA表达。通过免疫组化检测，MMP-13蛋白呈现出与MMP-13 mRNA相似的表达模式。在2种膀胱癌细胞系（RT4和T24）中也检测到MMP-13 mRNA和蛋白的表达。在这些细胞系中，肿瘤坏死因子-α强烈诱导MMP-13 mRNA表达。维甲酸和一种选择性p38抑制剂SB203580强烈抑制MMP-13 mRNA表达。我们的结果证明了MMP-13在人膀胱癌细胞体内和体外的表达，并提示MMP-13可能作为膀胱TCC转化和侵袭的一个标志物。
