Sharif Ali, Akhtar Muhammad Furqan, Akhtar Bushra, Saleem Ammara, Manan Maria, Shabbir Maryam, Ashraf Muneeb, Peerzada Sohaib, Ahmed Shoaib, Raza Moosa
Faculty of Pharmacy, the University of Lahore, Lahore, Pakistan.
Institute of Pharmacy, Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan.
EXCLI J. 2017 Apr 24;16:593-601. doi: 10.17179/excli2016-748. eCollection 2017.
Lack of data on safety of herbal medicines have endangered human health and life. The present study evaluated the genotoxic and mutagenic effect of to access the safety and usefulness of the medicinal plant. Aqua-methanolic and n-hexane extracts of were evaluated for the genotoxic potential using Ames assay and cytotoxicity was evaluated using MTT assay. Ames assay was conducted using two strains of TA-100 and TA-102 whereas MTT assay was performed on baby hamster kidney cell line BHK-21. Aqua-methanolic extract of exhibited significant mutagenicity when exposed to TA-102 strain with a mutagenic index of 50.66 and 54.74 at maximum dose 150 mg/plate. The extract was also mutagenic to TA-100 strain but to a lesser extent. M.I of n-hexane extract was 12.15 and 15.51 for TA-100 and TA-102 respectively. n-hexane extract was mutagenic but little difference was observed between results of two strains. Both extracts were found to be cytotoxic with an IC of 321.9 and 638.5 µg/mL for aqua-methanolic and n-hexane extracts respectively. On the basis of results it was concluded that aqua-methanolic and n-hexane extracts of possess mutagenic and cytotoxic potential. It is suggested to explore the plant further to evaluate its safety in rodents and other species.
草药安全性数据的缺失危及了人类健康和生命。本研究评估了[植物名称]的遗传毒性和致突变性，以确定该药用植物的安全性和实用性。使用艾姆斯试验评估了[植物名称]的水甲醇提取物和正己烷提取物的遗传毒性潜力，并使用MTT试验评估了细胞毒性。使用TA - 100和TA - 102两种菌株进行艾姆斯试验，而MTT试验则在幼仓鼠肾细胞系BHK - 21上进行。当[植物名称]的水甲醇提取物暴露于TA - 102菌株时，在最大剂量150 mg/平板下表现出显著的致突变性，突变指数分别为50.66和54.74。该提取物对TA - 100菌株也有致突变性，但程度较小。正己烷提取物对TA - 100和TA - 102的突变指数分别为12.15和15.51。正己烷提取物具有致突变性，但两种菌株的结果之间差异不大。两种提取物均具有细胞毒性，水甲醇提取物和正己烷提取物的IC50分别为321.9和638.5 µg/mL。根据结果得出结论，[植物名称]的水甲醇提取物和正己烷提取物具有致突变和细胞毒性潜力。建议进一步研究该植物，以评估其在啮齿动物和其他物种中的安全性。
