J Toxicol Sci. 2011 Oct;36(5):661-8. doi: 10.2131/jts.36.661.
The beneficial effects of Acanthopanax divaricatus var. albeofructus (ADA) extracts have been assessed by mutagenic and anti-mutagenic activities by Ames test. Mutation of Salmonella typhimurium strains TA 98, TA 100, TA1535, TA1537, and Escherichia coli WP2 uvr A was assayed in duplicates by the procedure of Maron and Ames in the presence or absence of S9 mix. As a result, ADA extracts were not mutagenic for S. typhimurium strains TA 98, TA 100, TA1535, TA1537, and E. coli by the Ames assay. Anti-mutagenic activity was assayed by the Ames mutagenicity assay using histidine mutant of S. typhimurium strains TA 98 and TA 100, using the plate-incorporation method. 2-Aminoanthrancene (2-AA), 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (AF-2), and sodium azide (NaN(3)) were used as the mutagens. ADA extracts showed a strong anti-mutagenic activity against 2-AA-induced mutagenesis which requires liver-metabolizing enzymes, and the same extract exhibited inhibitory effects on AF-2 and NaN(3)-induced mutagenesis in the absence of liver-metabolizing enzymes. The data indicate that ADA extracts contain anti-mutagenic activities against typical mutagens. The anti-mutagenic property of ADA provides additional health supplemental value to the other claimed therapeutic properties of the plant.
刺五加提取物的有益作用已通过 Ames 试验的致突变和抗突变活性进行了评估。采用 Maron 和 Ames 的程序，在存在或不存在 S9 混合物的情况下，用沙门氏菌 typhimurium 菌株 TA 98、TA 100、TA1535、TA1537 和大肠杆菌 WP2 uvr A 双重测定突变。结果表明，刺五加提取物对 Ames 试验中的沙门氏菌 typhimurium 菌株 TA 98、TA 100、TA1535、TA1537 和大肠杆菌无致突变性。采用平板掺入法，用 S. typhimurium 菌株 TA 98 和 TA 100 的组氨酸突变体，通过 Ames 致突变性试验测定抗突变活性。2-氨基蒽（2-AA）、2-（2-呋喃基）-3-（5-硝基-2-呋喃基）丙烯酰胺（AF-2）和叠氮化钠（NaN（3））用作诱变剂。刺五加提取物对需要肝代谢酶的 2-AA 诱导的致突变作用具有很强的抗突变活性，并且相同的提取物对 AF-2 和无肝代谢酶的 NaN（3）诱导的致突变作用具有抑制作用。数据表明，刺五加提取物含有针对典型诱变剂的抗突变活性。刺五加提取物的抗突变特性为该植物的其他声称的治疗特性提供了额外的健康补充价值。
