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墨西哥南部州牛群中具有公共卫生和兽医重要性的物种的分子鉴定

Molecular Identification of Species of Public Health and Veterinary Importance from Cattle in the South State of México.

作者信息

Zaragoza Bastida Adrian, Rivero Pérez Nallely, Valladares Carranza Benjamín, Isaac-Olivé Keila, Moreno Pérez Pablo, Sandoval Trujillo Horacio, Ramírez Durán Ninfa

机构信息

Facultad de Medicina, Universidad Autónoma del Estado de México, Paseo Tollocan/Jesús Carranza s/n, 50180 Toluca, MEX, Mexico.

Área Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuaria, Universidad Autónoma del Estado de Hidalgo, Av. Universidad Km 1, Ex-Hda. de Aquetzalpa, 43600 Tulancingo, HGO, Mexico.

出版信息

Can J Infect Dis Med Microbiol. 2017;2017:6094587. doi: 10.1155/2017/6094587. Epub 2017 Jun 14.

DOI:10.1155/2017/6094587
PMID:28694831
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5488153/
Abstract

genus causes a variety of zoonotic diseases. The best known example is the zoonotic tuberculosis due to . Much less is known about "nontuberculous mycobacteria (NTM)," which are also associated with infections in humans. The Mexican standard NOM-ZOO-031-1995 regulates the presence of in cattle; however, no regulation exists for the NTM species. The objective of this study was to isolate and identify nontuberculous mycobacteria species from cattle of local herds in the south region of the State of Mexico through the identification and detection of the 100 bp molecular marker in the 23S rRNA gene with subsequent sequencing of the 16S rRNA gene. Milk samples (35) and nasal exudate samples (68) were collected. From the 108 strains isolated, 39 were selected for identification. Thirteen strains isolated from nasal exudates amplified the 100 bp molecular marker and were identified as (six strains), (four strains), (two strains), and (one strain). Except , the other species identified are of public health and veterinary concern because they are pathogenic to humans, especially those with underlying medical conditions.

摘要

某属会引发多种人畜共患病。最广为人知的例子是由该属引起的人畜共患结核病。对于“非结核分枝杆菌(NTM)”人们了解得要少得多,它们也与人类感染有关。墨西哥标准NOM-ZOO-031-1995规定了牛群中该属的存在情况;然而,对于非结核分枝杆菌物种却没有相关规定。本研究的目的是通过鉴定和检测23S rRNA基因中的100bp分子标记并随后对16S rRNA基因进行测序,从墨西哥州南部地区本地牛群中分离并鉴定非结核分枝杆菌物种。采集了牛奶样本(35份)和鼻分泌物样本(68份)。从分离出的108株菌株中,选择了39株进行鉴定。从鼻分泌物中分离出的13株菌株扩增出了100bp分子标记,并被鉴定为某菌(6株)、某菌(4株)、某菌(2株)和某菌(1株)。除某菌外,鉴定出的其他物种都对公共卫生和兽医领域有影响,因为它们对人类致病,尤其是对有基础疾病的人。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/139c/5488153/615aff45d548/CJIDMM2017-6094587.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/139c/5488153/615aff45d548/CJIDMM2017-6094587.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/139c/5488153/615aff45d548/CJIDMM2017-6094587.001.jpg

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本文引用的文献

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