Versura P, Maltarello M C, Müller R M, Roomans G M
Institute of Clinical Electron Microscopy, University of Bologna, Italy.
Histochemistry. 1988;90(4):285-8. doi: 10.1007/BF00495972.
Chronic treatment of rats with reserpine, isoproterenol, or a combination of these two agents has been suggested as a means to produce an experimental animal model for the chronic exocrinopathy cystic fibrosis. The effect of these treatments on glycoconjugate distribution in rat submandibular gland acinar cells was investigated by quantitative lectin cytochemistry. Significant changes in wheat-germ agglutinin (WGA), soy bean agglutinin (SBA) and concanavalin A (Con A) binding sites in the mucus granules were observed, but peanut agglutinin (PNA) binding was not significantly affected. The quantitative changes in glycoconjugates in the acinar cells of the submandibular gland could be a possible explanation for the increased binding of calcium by the intracellular mucus noted in previous studies on these animal models.
有人提出，用利血平、异丙肾上腺素或这两种药物的组合对大鼠进行长期治疗，作为建立慢性外分泌病囊性纤维化实验动物模型的一种方法。通过定量凝集素细胞化学研究了这些处理对大鼠下颌下腺腺泡细胞中糖缀合物分布的影响。观察到黏液颗粒中麦胚凝集素（WGA）、大豆凝集素（SBA）和伴刀豆球蛋白A（Con A）结合位点有显著变化，但花生凝集素（PNA）结合未受显著影响。下颌下腺腺泡细胞中糖缀合物的定量变化可能是先前对这些动物模型研究中所指出的细胞内黏液钙结合增加的一个可能解释。
