Shane S M, Gyimah J E, Harrington K S, Snider T G
Vet Res Commun. 1985 Sep;9(4):269-87. doi: 10.1007/BF02215151.
Sporulated oocysts of Eimeria acervulina were administered orally to cage-housed broilers at a dose of 3.5 X 10(5) resulted in mild subclinical coccidiosis. Clostridium perfringens incorporated in feed at a level of 2.5 X 10(8) organisms/g. produced lesions characteristic of necrotic enteritis. Mortality of 8% (7/80) occurred in birds fed a ration inoculated with Cl. perfringens alone. Mortality of 35% (28/80) was observed in birds which received an oral dose of E. acervulina and which were fed simultaneously with a ration containing Cl. perfringens. Birds which were fed an inoculated ration two days after an oral dose of E. acervulina showed 41% (33/80) mortality. Birds which received an inoculated ration for two days before administration of an oral dose of E. acervulina demonstrated 18% mortality (15/80). Birds which were fed an inoculated ration four days after an oral dose of E. acervulina showed 10% mortality. Infection with E. acervulina reduced the pH of intestinal contents with a simultaneous depression in serum protein. A 39% increase in intestinal passage time from 178 to 248 minutes occurred on the fifth day after infection with E. acervulina. These experiments suggest that necrotic enteritis, attributed to proliferation of a toxigenic strain of Cl. perfringens, followed intestinal stasis and minimal lesions induced by mild intestinal coccidiosis.
将堆型艾美耳球虫的孢子化卵囊以3.5×10⁵的剂量口服给笼养肉鸡，导致轻度亚临床球虫病。将产气荚膜梭菌以2.5×10⁸个菌/克的水平添加到饲料中，产生了坏死性肠炎的特征性病变。仅喂食接种了产气荚膜梭菌饲料的鸡群死亡率为8%（7/80）。口服堆型艾美耳球虫且同时喂食含产气荚膜梭菌饲料的鸡群死亡率为35%（28/80）。在口服堆型艾美耳球虫两天后喂食接种饲料的鸡群死亡率为41%（33/80）。在口服堆型艾美耳球虫前喂食接种饲料两天的鸡群死亡率为18%（15/80）。在口服堆型艾美耳球虫四天后喂食接种饲料的鸡群死亡率为10%。堆型艾美耳球虫感染降低了肠道内容物的pH值，同时血清蛋白降低。感染堆型艾美耳球虫后第五天，肠道通过时间从178分钟增加到248分钟，增加了39%。这些实验表明，由产气荚膜梭菌产毒株增殖引起的坏死性肠炎继发于肠道淤滞和轻度肠道球虫病引起的最小病变。
