Klimov A I, Schräder C, Herrmann B, Seidel W, Döhner L
Acta Virol. 1985 Dec;29(6):466-74.
Gene variability of all influenza A virus strains (H3N2) isolated in the G.D.R. during the epidemic seasons of 1970-1978 was investigated by cRNA:vRNA hybridization. From 1970 through 1975 a gradual smooth variability of the majority of genes and moderate heterogeneity in gene homology of the isolates were observed. From 1975 through 1977 the genome variability was more profound and the isolates differed from one another in gene homology. In 1978 the variability became less pronounced again. Quantitative evaluation of the variability for individual genes gave the following results: 0.4% nucleotides per year for genes, 1, 2, 3 (P proteins), 1.4% for gene 4 (HA), 0.1% for gene 5 (NP), 1.0% for gene 6 (NA), 0.1% for gene 7 (M) and 0.4 for gene 8 (NS).
通过cRNA:vRNA杂交技术，对1970 - 1978年流行季节期间在德意志民主共和国分离出的所有甲型流感病毒株（H3N2）的基因变异性进行了研究。1970年至1975年期间，观察到大多数基因逐渐平稳变异，分离株的基因同源性存在中度异质性。1975年至1977年，基因组变异性更为显著，分离株在基因同源性上彼此不同。1978年，变异性再次变得不那么明显。对各个基因变异性的定量评估得出以下结果：基因1、2、3（P蛋白）每年的核苷酸变异率为0.4%，基因4（HA）为1.4%，基因5（NP）为0.1%，基因6（NA）为1.0%，基因7（M）为0.1%，基因8（NS）为0.4%。
