富含层粘连蛋白-111 的纤维蛋白水凝胶用于骨骼肌再生。
Laminin-111 enriched fibrin hydrogels for skeletal muscle regeneration.
机构信息
Department of Biomedical Engineering, Parks College of Engineering, Aviation, and Technology, Saint Louis University, USA.
Department of Biomedical Engineering, Parks College of Engineering, Aviation, and Technology, Saint Louis University, USA.
出版信息
Biomaterials. 2017 Oct;141:233-242. doi: 10.1016/j.biomaterials.2017.07.003. Epub 2017 Jul 3.
Laminin (LM)-111 supplementation has improved muscle regeneration in several models of disease and injury. This study investigated a novel hydrogel composed of fibrinogen and LM-111. Increasing LM-111 concentration (50-450 μg/mL) in fibrin hydrogels resulted in highly fibrous scaffolds with progressively thinner interlaced fibers. Rheological testing showed that all hydrogels had viscoelastic behavior and the Young's modulus ranged from 2-6KPa. CC myobalsts showed a significant increase in VEGF production and decrease in IL-6 production on LM-111 enriched fibrin hydrogels as compared to pure fibrin hydrogels on day 4. Western blotting results showed a significant increase in MyoD and desmin protein quantity but a significant decrease in myogenin protein quantity in myoblasts cultured on the LM-111 (450 μg/mL) enriched fibrin hydrogel. Combined application of electromechanical stimulation significantly enhanced the production of VEGF and IGF-1 from myoblast seeded fibrin-LM-111 hydrogels. Taken together, these observations offer an important first step toward optimizing a tissue engineered constructs for skeletal muscle regeneration.
层粘连蛋白 (LM)-111 的补充已改善了几种疾病和损伤模型中的肌肉再生。本研究调查了一种由纤维蛋白原和 LM-111 组成的新型水凝胶。在纤维蛋白水凝胶中增加 LM-111 浓度（50-450μg/mL），会导致纤维越来越细的高度纤维状支架。流变学测试表明，所有水凝胶均具有粘弹性，杨氏模量范围为 2-6KPa。与纯纤维蛋白水凝胶相比，CC 成肌细胞在富含 LM-111 的纤维蛋白水凝胶上培养 4 天时，VEGF 的产生显著增加，IL-6 的产生显著减少。Western blot 结果表明，在富含 LM-111（450μg/mL）的纤维蛋白水凝胶上培养的成肌细胞中，MyoD 和结蛋白蛋白的数量显著增加，而 myogenin 蛋白的数量显著减少。电机械刺激的联合应用显著增强了富含纤维蛋白-LM-111 的成肌细胞水凝胶中 VEGF 和 IGF-1 的产生。综上所述，这些观察结果为优化用于骨骼肌再生的组织工程构建体提供了重要的第一步。
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