Fan Chaofeng, Zhai Huijie, Wang Huifang, Yue Yafei, Zhang Minghu, Li Jinghui, Wen Shaozhe, Guo Ganggang, Zeng Yawen, Ni Zhongfu, You Mingshan
State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China.
National Plant Gene Research Centre, Beijing, 100193, China.
BMC Plant Biol. 2017 Jul 11;17(1):122. doi: 10.1186/s12870-017-1067-6.
Grain protein concentration (GPC) is a major determinant of quality in barley (Hordeum vulgare L.). Breeding barley cultivars with high GPC has practical value for feed and food properties. The aim of the present study was to identify quantitative trait loci (QTLs) for GPC that could be detected under multiple environments.
A population of 190 recombinant inbred lines (RILs) deriving from a cross between Chinese landrace ZGMLEL with high GPC (> 20%) and Australian cultivar Schooner was used for linkage and QTL analyses. The genetic linkage map spanned 2353.48 cM in length with an average locus interval of 2.33 cM. GPC was evaluated under six environments for the RIL population and the two parental lines. In total, six environmentally stable QTLs for GPC were detected on chromosomes 2H (1), 4H (1), 6H (1), and 7H (3) and the increasing alleles were derived from ZGMLEL. Notably, the three QTLs on chromosome 7H (QGpc.ZiSc-7H.1, QGpc.ZiSc-7H.2, and QGpc.ZiSc-7H.3) that linked in coupling phase were firstly identified. Moreover, the genetic effects of stable QTLs on chromosomes 2H, 6H and 7H were validated using near isogenic lines (NILs).
Collectively, the identified QTLs expanded our knowledge about the genetic basis of GPC in barley and could be selected to develop cultivars with high grain protein concentration.
籽粒蛋白质含量（GPC）是大麦（Hordeum vulgare L.）品质的主要决定因素。培育高GPC的大麦品种对饲料和食品特性具有实际价值。本研究的目的是鉴定在多种环境下都能检测到的GPC数量性状位点（QTL）。
利用中国高GPC（>20%）地方品种ZGMLEL与澳大利亚品种Schooner杂交产生的190个重组自交系（RIL）群体进行连锁和QTL分析。遗传连锁图谱全长2353.48 cM，平均位点间隔为2.33 cM。对RIL群体和两个亲本系在六种环境下评估GPC。总共在2H（1个）、4H（1个）、6H（1个）和7H（3个）染色体上检测到六个环境稳定的GPC QTL，增效等位基因来自ZGMLEL。值得注意的是，首次鉴定出在染色体7H上呈耦合相连锁的三个QTL（QGpc.ZiSc - 7H.1、QGpc.ZiSc - 7H.2和QGpc.ZiSc - 7H.3）。此外，利用近等基因系（NIL）验证了2H、6H和7H染色体上稳定QTL的遗传效应。
总体而言，鉴定出的QTL扩展了我们对大麦GPC遗传基础的认识，可用于选育高籽粒蛋白质含量的品种。
