Alptekin Burcu, Erfatpour Mohammad, Mangel Dylan, Pauli Duke, Blake Tom, Turner Hannah, Lachowiec Jennifer, Sherman Jamie, Fischer Andreas
Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717 USA.
Present Address: Department of Bacteriology, University of Wisconsin, Madison, WI 53706 USA.
Mol Breed. 2022 Sep 22;42(10):59. doi: 10.1007/s11032-022-01331-7. eCollection 2022 Oct.
Malt barley ( L.) is an important cash crop with stringent grain quality standards. Timing of the switch from vegetative to reproductive growth and timing of whole-plant senescence and nutrient remobilization are critical for cereal grain yield and quality. Understanding the genetic variation in genes associated with these developmental traits can streamline genotypic selection of superior malt barley germplasm. Here, we determined the effects of allelic variation in three genes encoding a glycine-rich RNA-binding protein (GR-RBP1) and two NAC transcription factors (NAM1 and NAM2) on malt barley agronomics and quality using previously developed markers for and and a novel marker for . Based on a single-nucleotide polymorphism (SNP) in the first intron, the utilized marker differentiates alleles of low-grain protein variety 'Karl' and of higher protein variety 'Lewis'. We demonstrate that the selection of favorable alleles for each gene impacts heading date, senescence timing, grain size, grain protein concentration, and malt quality. Specifically, combining 'Karl' alleles for the two genes with the 'Lewis' allele extends grain fill duration, increases the percentage of plump kernels, decreases grain protein, and provides malt quality stability. Molecular markers for these genes are therefore highly useful tools in malt barley breeding.
The online version contains supplementary material available at 10.1007/s11032-022-01331-7.
麦芽大麦（L.）是一种重要的经济作物，有着严格的谷物品质标准。从营养生长向生殖生长转变的时间以及整株衰老和养分再分配的时间，对于谷物产量和品质至关重要。了解与这些发育性状相关基因的遗传变异，有助于优化优质麦芽大麦种质的基因型选择。在此，我们利用先前开发的用于 和 的标记以及一个新开发的用于 的标记，确定了编码富含甘氨酸的RNA结合蛋白（GR-RBP1）的三个基因和两个NAC转录因子（NAM1和NAM2）的等位基因变异对麦芽大麦农艺学和品质的影响。基于第一个内含子中的单核苷酸多态性（SNP），所使用的标记区分了低谷物蛋白品种“卡尔”和高蛋白品种“刘易斯”的 等位基因。我们证明，对每个基因选择有利等位基因会影响抽穗期、衰老时间、籽粒大小、籽粒蛋白浓度和麦芽品质。具体而言，将两个 基因的“卡尔”等位基因与“刘易斯”的 等位基因相结合，可延长灌浆持续时间，增加饱满籽粒百分比，降低籽粒蛋白含量，并提供麦芽品质稳定性。因此，这些基因的分子标记是麦芽大麦育种中非常有用的工具。
在线版本包含可在10.1007/s11032-022-01331-7获取的补充材料。
