• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

生物膜干涉术可预测用于恶性疟原虫富含组氨酸蛋白2的单克隆抗体对的酶联免疫吸附测定性能。

Biolayer interferometry predicts ELISA performance of monoclonal antibody pairs for Plasmodium falciparum histidine-rich protein 2.

作者信息

Markwalter C F, Jang I K, Burton R A, Domingo G J, Wright D W

机构信息

Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA.

PATH, Seattle, WA 98121, USA.

出版信息

Anal Biochem. 2017 Oct 1;534:10-13. doi: 10.1016/j.ab.2017.07.010. Epub 2017 Jul 8.

DOI:10.1016/j.ab.2017.07.010
PMID:28698001
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5552614/
Abstract

Predicting antibody pair performance in a sandwich format streamlines development of antibody-based diagnostics and laboratory research tools, such as enzyme-linked immunosorbent assays (ELISAs) and lateral flow immunoassays (LFAs). We have evaluated panels of monoclonal antibodies against the malarial parasite biomarker Plasmodium falciparum histidine rich protein 2 (HRP2), including 9 new monoclonal antibodies, using biolayer interferometry (BLI) and screened antibody pairs in a checkerboard ELISA. This study showed BLI predicts antibody pair ELISA performance for HRP2. Pairs that included capture antibodies with low off-rate constants and detection antibodies with high on-rate constants performed best in an ELISA format.

摘要

预测夹心形式下抗体对的性能可简化基于抗体的诊断方法和实验室研究工具（如酶联免疫吸附测定法（ELISA）和侧向流动免疫测定法（LFA））的开发。我们使用生物层干涉术（BLI）评估了针对疟原虫生物标志物恶性疟原虫富含组氨酸蛋白2（HRP2）的单克隆抗体组，其中包括9种新的单克隆抗体，并在棋盘式ELISA中筛选了抗体对。这项研究表明，BLI可预测针对HRP2的抗体对ELISA性能。在ELISA形式中，包含解离速率常数低的捕获抗体和结合速率常数高的检测抗体的抗体对表现最佳。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5aca/5552614/df4a83b05068/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5aca/5552614/20fc6dcc802f/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5aca/5552614/df4a83b05068/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5aca/5552614/20fc6dcc802f/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5aca/5552614/df4a83b05068/gr1.jpg

相似文献

1
Biolayer interferometry predicts ELISA performance of monoclonal antibody pairs for Plasmodium falciparum histidine-rich protein 2.生物膜干涉术可预测用于恶性疟原虫富含组氨酸蛋白2的单克隆抗体对的酶联免疫吸附测定性能。
Anal Biochem. 2017 Oct 1;534:10-13. doi: 10.1016/j.ab.2017.07.010. Epub 2017 Jul 8.
2
Quantification of Histidine-Rich Protein 3 of Plasmodium falciparum.恶性疟原虫富含组氨酸蛋白3的定量分析
Monoclon Antib Immunodiagn Immunother. 2018 Apr;37(2):87-90. doi: 10.1089/mab.2017.0068. Epub 2018 Apr 10.
3
Diagnostic potential of monoclonal antibodies developed against C-terminal polypeptide of P. falciparum Histidine Rich Protein2 (PfHRP2) in malaria infected patients from India.抗恶性疟原虫富组氨酸蛋白 2（PfHRP2）C 端多肽单克隆抗体在印度疟疾感染患者中的诊断潜力。
Pathog Glob Health. 2017 Sep;111(6):297-305. doi: 10.1080/20477724.2017.1358846. Epub 2017 Aug 4.
4
Novel monoclonal antibody against truncated C terminal region of Histidine Rich Protein2 (PfHRP2) and its utility for the specific diagnosis of malaria caused by Plasmodium falciparum.针对富含组氨酸蛋白2（PfHRP2）截短C末端区域的新型单克隆抗体及其在恶性疟原虫引起的疟疾特异性诊断中的应用。
Exp Parasitol. 2015 Mar;150:56-66. doi: 10.1016/j.exppara.2015.01.001. Epub 2015 Jan 12.
5
Simultaneous capture and sequential detection of two malarial biomarkers on magnetic microparticles.在磁性微粒上同时捕获和顺序检测两种疟疾生物标志物。
Talanta. 2016 Dec 1;161:443-449. doi: 10.1016/j.talanta.2016.08.078. Epub 2016 Aug 30.
6
Immunogenicity of synthetic peptides derived from Plasmodium falciparum proteins.源自恶性疟原虫蛋白质的合成肽的免疫原性。
Exp Parasitol. 2006 Aug;113(4):227-34. doi: 10.1016/j.exppara.2006.01.007. Epub 2006 Mar 2.
7
Purification of native histidine-rich protein 2 (nHRP2) from Plasmodium falciparum culture supernatant, infected RBCs, and parasite lysate.从恶性疟原虫培养液上清液、感染红细胞和虫体裂解液中纯化天然组氨酸丰富蛋白 2（nHRP2）。
Malar J. 2021 Oct 17;20(1):405. doi: 10.1186/s12936-021-03946-1.
8
Production and characterization of specific monoclonal antibodies binding the Plasmodium falciparum diagnostic biomarker, histidine-rich protein 2.结合恶性疟原虫诊断生物标志物富含组氨酸蛋白2的特异性单克隆抗体的制备与特性分析
Malar J. 2014 Jul 18;13:277. doi: 10.1186/1475-2875-13-277.
9
Evidence for histidine-rich protein 2 immune complex formation in symptomatic patients in Southern Zambia.赞比亚南部症状患者中组氨酸丰富蛋白 2 免疫复合物形成的证据。
Malar J. 2018 Jul 9;17(1):256. doi: 10.1186/s12936-018-2400-8.
10
Development, purification, and characterization of monoclonal antibodies against recombinant histidine-rich protein 3 of Plasmodium falciparum.恶性疟原虫重组富含组氨酸蛋白3单克隆抗体的研制、纯化及特性鉴定
Monoclon Antib Immunodiagn Immunother. 2013 Oct;32(5):341-8. doi: 10.1089/mab.2013.0018.

引用本文的文献

1
Epitope Profiling of SARS-CoV-2 Spike Antigen Provides a Novel Strategy for Developing ELISAs Specific for Different Spike Protein Variants in Bivalent Vaccine Formulations.SARS-CoV-2刺突抗原的表位分析为开发针对二价疫苗配方中不同刺突蛋白变体的特异性ELISA提供了一种新策略。
Vaccines (Basel). 2025 Jul 26;13(8):794. doi: 10.3390/vaccines13080794.
2
Liquid Redox Probe-Free Plastic Antibody Development for Malaria Biomarker Recognition.用于疟疾生物标志物识别的无液体氧化还原探针塑料抗体开发。
ACS Omega. 2024 Jul 15;9(30):33130-33139. doi: 10.1021/acsomega.4c04543. eCollection 2024 Jul 30.
3
Fully synthetic phosphorylated Tau181, Tau217, and Tau231 calibrators for Alzheimer's disease diagnosis.

本文引用的文献

1
Assessing the impact of next-generation rapid diagnostic tests on Plasmodium falciparum malaria elimination strategies.评估新一代快速诊断检测对恶性疟原虫疟疾消除策略的影响。
Nature. 2015 Dec 3;528(7580):S94-101. doi: 10.1038/nature16040.
2
Submicroscopic and asymptomatic Plasmodium falciparum and Plasmodium vivax infections are common in western Thailand - molecular and serological evidence.泰国西部常见微小隐伏的恶性疟原虫和间日疟原虫感染——分子学与血清学证据
Malar J. 2015 Feb 25;14:95. doi: 10.1186/s12936-015-0611-9.
3
Measuring protein-protein and protein-nucleic Acid interactions by biolayer interferometry.
用于阿尔茨海默病诊断的全合成磷酸化Tau181、Tau217和Tau231校准品。
Front Aging Neurosci. 2024 Jan 15;15:1340706. doi: 10.3389/fnagi.2023.1340706. eCollection 2023.
4
Label-Free Optical Resonator-Based Biosensors.无标记光学共振生物传感器。
Sensors (Basel). 2020 Oct 19;20(20):5901. doi: 10.3390/s20205901.
5
Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects.基于等离子体激元、共振和干涉效应的光纤和波导直接光学检测生物传感器相关方法的批判性评估。
Anal Bioanal Chem. 2020 May;412(14):3317-3349. doi: 10.1007/s00216-020-02581-0. Epub 2020 Apr 20.
6
Multiplex Human Malaria Array: Quantifying Antigens for Malaria Rapid Diagnostics.多重人类疟疾检测芯片：疟疾快速诊断学中的抗原定量。
Am J Trop Med Hyg. 2020 Jun;102(6):1366-1369. doi: 10.4269/ajtmh.19-0763.
7
Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA.用于改进基于纸的酶联免疫吸附测定中蛋白质固定的原子转移自由基聚合物改性纸。
BMC Chem. 2019 Aug 22;13(1):110. doi: 10.1186/s13065-019-0622-7. eCollection 2019 Dec.
8
Rapid Antibody Selection Using Surface Plasmon Resonance for High-Speed and Sensitive Hazelnut Lateral Flow Prototypes.基于表面等离子体共振的快速抗体筛选，用于高速灵敏榛子侧向流原型。
Biosensors (Basel). 2018 Dec 14;8(4):130. doi: 10.3390/bios8040130.
9
Simultaneous Quantification of Antigens and Host Factor C-Reactive Protein in Asymptomatic Individuals with Confirmed Malaria by Use of a Novel Multiplex Immunoassay.利用新型多重免疫分析法在无症状的确诊疟疾个体中同时定量检测抗原和宿主因子 C-反应蛋白。
J Clin Microbiol. 2019 Jan 2;57(1). doi: 10.1128/JCM.00948-18. Print 2019 Jan.
10
Evidence for histidine-rich protein 2 immune complex formation in symptomatic patients in Southern Zambia.赞比亚南部症状患者中组氨酸丰富蛋白 2 免疫复合物形成的证据。
Malar J. 2018 Jul 9;17(1):256. doi: 10.1186/s12936-018-2400-8.
利用生物膜干涉术测量蛋白质-蛋白质和蛋白质-核酸相互作用。
Curr Protoc Protein Sci. 2015 Feb 2;79:19.25.1-19.25.26. doi: 10.1002/0471140864.ps1925s79.
4
Asymptomatic malaria infections: detectability, transmissibility and public health relevance.无症状疟疾感染：检出率、传染性和公共卫生相关性。
Nat Rev Microbiol. 2014 Dec;12(12):833-40. doi: 10.1038/nrmicro3364. Epub 2014 Oct 20.
5
The role of asymptomatic P. falciparum parasitaemia in the evolution of antimalarial drug resistance in areas of seasonal transmission.无症状疟原虫血症在季节性传播地区抗疟药物耐药性演变中的作用。
Drug Resist Updat. 2013 Feb-Apr;16(1-2):1-9. doi: 10.1016/j.drup.2013.02.001. Epub 2013 Mar 17.
6
Malaria rapid diagnostic tests in endemic settings.疟疾快速诊断检测在流行地区的应用。
Clin Microbiol Infect. 2013 May;19(5):399-407. doi: 10.1111/1469-0691.12151. Epub 2013 Feb 25.
7
Transcription and expression of Plasmodium falciparum histidine-rich proteins in different stages and strains: implications for rapid diagnostic tests.恶性疟原虫组氨酸丰富蛋白在不同阶段和株系中的转录和表达：对快速诊断检测的影响。
PLoS One. 2011;6(7):e22593. doi: 10.1371/journal.pone.0022593. Epub 2011 Jul 22.
8
Label-free detection of biomolecular interactions using BioLayer interferometry for kinetic characterization.使用生物层干涉术进行动力学表征的生物分子相互作用的无标记检测。
Comb Chem High Throughput Screen. 2009 Sep;12(8):791-800. doi: 10.2174/138620709789104915.
9
Rational screening of antibodies and design of sandwich enzyme linked immunosorbant assay on the basis of a kinetic model.基于动力学模型的抗体合理筛选及夹心酶联免疫吸附测定法的设计
J Biosci Bioeng. 2008 Mar;105(3):261-72. doi: 10.1263/jbb.105.261.
10
Genetic diversity of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and its effect on the performance of PfHRP2-based rapid diagnostic tests.恶性疟原虫富含组氨酸蛋白2（PfHRP2）的遗传多样性及其对基于PfHRP2的快速诊断检测性能的影响。
J Infect Dis. 2005 Sep 1;192(5):870-7. doi: 10.1086/432010. Epub 2005 Jul 21.