Markwalter C F, Jang I K, Burton R A, Domingo G J, Wright D W
Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA.
PATH, Seattle, WA 98121, USA.
Anal Biochem. 2017 Oct 1;534:10-13. doi: 10.1016/j.ab.2017.07.010. Epub 2017 Jul 8.
Predicting antibody pair performance in a sandwich format streamlines development of antibody-based diagnostics and laboratory research tools, such as enzyme-linked immunosorbent assays (ELISAs) and lateral flow immunoassays (LFAs). We have evaluated panels of monoclonal antibodies against the malarial parasite biomarker Plasmodium falciparum histidine rich protein 2 (HRP2), including 9 new monoclonal antibodies, using biolayer interferometry (BLI) and screened antibody pairs in a checkerboard ELISA. This study showed BLI predicts antibody pair ELISA performance for HRP2. Pairs that included capture antibodies with low off-rate constants and detection antibodies with high on-rate constants performed best in an ELISA format.
预测夹心形式下抗体对的性能可简化基于抗体的诊断方法和实验室研究工具（如酶联免疫吸附测定法（ELISA）和侧向流动免疫测定法（LFA））的开发。我们使用生物层干涉术（BLI）评估了针对疟原虫生物标志物恶性疟原虫富含组氨酸蛋白2（HRP2）的单克隆抗体组，其中包括9种新的单克隆抗体，并在棋盘式ELISA中筛选了抗体对。这项研究表明，BLI可预测针对HRP2的抗体对ELISA性能。在ELISA形式中，包含解离速率常数低的捕获抗体和结合速率常数高的检测抗体的抗体对表现最佳。
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