Diagnostics, PATH, Seattle, Washington.
Quansys Biosciences, Logan, Utah.
Am J Trop Med Hyg. 2020 Jun;102(6):1366-1369. doi: 10.4269/ajtmh.19-0763.
Malaria antigen detection through rapid diagnostic tests (RDTs) is widely used to diagnose malaria and estimate prevalence. To support more sensitive next-generation RDT development and screen asymptomatic malaria, we developed and evaluated the Q-Plex Human Malaria Array (Quansys Biosciences, Logan, UT), which quantifies the antigens commonly used in RDTs--specific histidine-rich protein 2 (HRP2), specific lactate dehydrogenase ( LDH), specific LDH ( LDH), and Pan malaria lactate dehydrogenase (Pan LDH), and human C-reactive protein (CRP), a biomarker of severity in malaria. At threshold levels yielding 99.5% or more diagnostic specificity, diagnostic sensitivities against polymerase chain reaction-confirmed malaria for HRP2, LDH, LDH, and Pan LDH were 92.7%, 71.5%, 46.1%, and 83.8%, respectively. culture strains and samples from Peru indicated that HRP2 and LDH combined improves detection of parasites with and deletions. This array can be used for antigen-based malaria screening and detecting deletion mutants of .
通过快速诊断检测（RDT）检测疟原抗原被广泛用于诊断疟疾和估计流行率。为了支持更敏感的下一代 RDT 开发和筛查无症状疟疾，我们开发并评估了 Q-Plex 人类疟疾阵列（Quansys Biosciences，Logan，UT），该阵列定量了 RDT 中常用的抗原 - 特异性富含组氨酸蛋白 2（HRP2），特异性乳酸脱氢酶（LDH），特异性乳酸脱氢酶（ LDH）和泛疟疾乳酸脱氢酶（Pan LDH），以及人类 C 反应蛋白（CRP），这是疟疾严重程度的生物标志物。在产生 99.5%或更高诊断特异性的阈值水平下，针对聚合酶链反应证实的疟疾的 HRP2、LDH、 LDH 和 Pan LDH 的诊断敏感性分别为 92.7%、71.5%、46.1%和 83.8%。培养株和来自秘鲁的样本表明，HRP2 和 LDH 联合提高了对 和 删除突变体的寄生虫检测。该阵列可用于基于抗原的疟疾筛查和检测 删除突变体。
