Arnold Nicole, Messaoudi Ilhem
Graduate Program in Microbiology, University of California, Riverside, CA, USA.
Department of Molecular Biology and Biochemistry, University of California-Irvine, Irvine, CA, 92697, USA.
Virus Res. 2017 Jun 15;238:226-235. doi: 10.1016/j.virusres.2017.07.004. Epub 2017 Jul 8.
Varicella zoster virus (VZV) causes varicella (chickenpox) during acute infection. Several studies have shown that T cells are early and preferential targets of VZV infection that play a critical role in disseminating VZV in to the skin and ganglia. However, the transcriptional changes that occur in VZV-infected T cells remain unclear due to limited access to clinical samples and robust translational animal models. In this study, we used a nonhuman primate model of VZV infection where rhesus macaques are infected with the closely related Simian Varicella Virus (SVV) to provide novel insights into VZV-T cell interactions. RNA sequencing of bronchial alveolar lavage-resident T cells isolated from infected rhesus macaques show that SVV infection alters expression of genes important for regulation of gene expression, cell cycle progression, metabolism, and antiviral immunity. These data provide insight into cellular processes that may support viral replication, facilitate SVV dissemination, and evade host defense.
水痘带状疱疹病毒（VZV）在急性感染期间会引发水痘（鸡痘）。多项研究表明，T细胞是VZV感染的早期且优先靶向目标，在将VZV传播至皮肤和神经节过程中发挥关键作用。然而，由于获取临床样本有限且缺乏强大的转化动物模型，VZV感染的T细胞中发生的转录变化仍不清楚。在本研究中，我们使用了VZV感染的非人灵长类动物模型，即恒河猴感染密切相关的猴水痘病毒（SVV），以提供关于VZV与T细胞相互作用的新见解。对从受感染恒河猴分离的支气管肺泡灌洗驻留T细胞进行RNA测序表明，SVV感染会改变对基因表达调控、细胞周期进程、代谢和抗病毒免疫重要的基因表达。这些数据为可能支持病毒复制、促进SVV传播并逃避宿主防御的细胞过程提供了见解。
